Biomaterial Database

Nuclear magnetic resonance investigation of the freezing of water in rat kidney tissues. 1978

V V Morariu, and I C Kiricuta, and C F Hazlewood

The proton linewidth and the integral of the absorption signal of water in rat kidney tissue have been measured at sub-zero temperatures. The water, fresh or stored at +4 degrees C for 24--48 h, shows a supercooling effect down to -10 degrees C to -14 degrees C. At -20 degrees C, 10--20% of the total tissue water remains unfrozen. The supercooling effect is diminished by prior storage at positive temperatures but completely eliminated by storage at several degrees below 0 degrees C. This suggests that the freezing behavior of tissue water varies with change in the physiological state of the tissue. Tissue samples stored at positive temperatures were observed to be more vulnerable than fresh tissue to freezing damage. Storage at several degrees below 0 degrees C for the same period of time appeared to be even more damaging.

UI	MeSH Term	Description	Entries
D007424	Intracellular Fluid	The fluid inside CELLS.	Fluid, Intracellular,Fluids, Intracellular,Intracellular Fluids
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D009682	Magnetic Resonance Spectroscopy	Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).	In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D012034	Refrigeration	The mechanical process of cooling.	Cold Chain,Chain, Cold,Chains, Cold,Cold Chains
D001826	Body Fluids	Liquid components of living organisms.	Body Fluid,Fluid, Body,Fluids, Body
D001834	Body Water	Fluids composed mainly of water found within the body.	Water, Body
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D014021	Tissue Preservation	The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).	Preservation, Tissue,Preservations, Tissue,Tissue Preservations