-transparent.png){kind=logo}
The role of both endotoxin and neutrophils in the development of acute lung injury continues to be debated. We hypothesized that early in the course of the development of the adult respiratory distress syndrome (ARDS) circulating neutrophils could be primed by endotoxin and that subsequent stimulated responses could be enhanced. Accordingly, neutrophils were isolated from patients at risk for and with ARDS. Unstimulated neutrophils from these patients neither produced nor were primed for superoxide production. Whereas phorbol myristate acetate-stimulated superoxide production was preserved, indicating that the cells were capable of a response, patient neutrophils produced less superoxide than did cells from normal subjects when primed with endotoxin (lipopolysaccharide [LPS]) and stimulated with formyl-methionyl-leucine-phenylalanine (FMLP), suggesting that there was a defect in the signal transduction mechanism for LPS. This was confirmed by the finding that patient neutrophils also had both decreased baseline CD14 expression and less CD14 upregulation after LPS stimulation compared with neutrophils from normal subjects. The mechanisms that could account for the decreased CD14 expression were studied in vitro. Neutrophils from normal subjects both upregulate CD14 in response to LPS and shed CD14 over time, suggesting that in patients CD14 receptors could have been previously upregulated and shed. In addition, there is an association between CD14 expression and retention such that normal LPS-stimulated neutrophils which are not retained in a filtration system have decreased CD14 expression. Thus, in patients, those PMN most responsive to LPS could be preferentially sequestered and not available in the circulation for study.
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
January 1993,
The British journal of surgery,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
July 1990,
British journal of hospital medicine,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
March 1994,
Chest,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
January 2002,
The European journal of surgery = Acta chirurgica,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
May 1981,
The New England journal of medicine,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
September 1989,
Mayo Clinic proceedings,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
January 1984,
Critical care medicine,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
January 2000,
Vutreshni bolesti,
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
November 1993,
New horizons (Baltimore, Md.),
P E Parsons, and
M M Gillespie, and
E E Moore, and
F A Moore, and
G S Worthen
September 1993,
Zhonghua nei ke za zhi,
