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It has been suggested that CD5+ B cells are closely related to the pathogenesis of autoimmune disease or chronic lymphocytic leukaemia, whereas the origin and physiological role of CD5+ B cells remain controversial. To study the molecular differences between CD5+ and CD5- B cells in terms of immunoglobulin gene structure, we sorted both subsets from new-born cord blood and analysed the complementarity determining region (CDR)-3 profiles of the immunoglobulin heavy chain (IgH) gene. The CDR-3 sequences from both CD5+ and CD5- B cells represented the same incidence and length of N-region, and the same usage of D and JH segments. When translated into amino acids, 24 of 32 clones (75%) and 25 of 37 clones (65.8%) from their respective subset were productive, and the composition of the deduced amino acids were similar between both subsets. These data suggest that CD5+ B cells are not a distinct lineage presenting a biased immunoglobulin repertoire in B cells.
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
March 1992,
The Journal of clinical investigation,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
January 1996,
BioTechniques,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
August 1984,
Proceedings of the National Academy of Sciences of the United States of America,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
November 1996,
Immunology,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
January 2004,
Journal of molecular recognition : JMR,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
August 1994,
The Journal of experimental medicine,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
November 1999,
Biochemistry,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
June 2000,
The Journal of experimental medicine,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
March 1999,
Immunotechnology : an international journal of immunological engineering,
H Kiyoi, and
K Naito, and
R Ohno, and
T Naoe
April 1992,
The Journal of clinical investigation,
