Biomaterial Database

Standardization of allergenic extracts by basophil histamine release. 1995

T R Kordash, and L L Freshwater, and M J Amend

Miles Inc., Allergy Products, Spokane, WA 99207, USA.

BACKGROUND Allergenic extracts are standardized by using the ID50EAL (Intradermal Dilution for 50 mm sum of Erythema Determines the Allergy Unit) skin test technique to assign allergy units to reference preparations. When new batches of extracts are manufactured, they are compared with the reference by RAST inhibition or other in vitro techniques. This study was designed to determine whether basophil histamine release might be an additional useful in vitro test for the standardization of allergenic extracts. METHODS Basophil histamine release, skin tests, and RAST were compared using several different allergenic extracts at many different strengths. Allergy units were calculated using skin test results and basophil histamine release. RESULTS Basophil histamine release, skin tests, and RAST correlated well (P < .01). Basophil histamine release-derived allergy units and skin test-derived allergy units were highly correlated (P < .01). CONCLUSIONS Basophil histamine release appears to be a useful tool for the standardization of allergenic extracts.

UI	MeSH Term	Description	Entries
D007159	Immunologic Tests	Immunologic techniques involved in diagnosis.	Diagnosis, Immunological,Immunodiagnosis,Diagnosis, Immunologic,Immunologic Diagnosis,Immunologic Test,Immunological Tests,Tests, Immunologic,Diagnoses, Immunologic,Diagnoses, Immunological,Immunodiagnoses,Immunologic Diagnoses,Immunological Diagnoses,Immunological Diagnosis,Immunological Test,Test, Immunologic,Test, Immunological,Tests, Immunological
D011852	Radioallergosorbent Test	An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.	Radioimmunosorbent Assay of Allergens,Allergens Radioimmunosorbent Assay,Allergens Radioimmunosorbent Assays,Radioallergosorbent Tests,Test, Radioallergosorbent,Tests, Radioallergosorbent
D006636	Histamine Release	The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.	Histamine Liberation,Histamine Liberations,Histamine Releases
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000485	Allergens	Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).	Allergen
D001491	Basophils	Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.	Basophil
D012882	Skin Tests	Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.	Skin Test,Test, Skin,Tests, Skin