-transparent.png){kind=logo}
In order to gain knowledge on the interaction of lysosomes with proteins, we have assessed the equilibrium densities of the lysosomal membrane and matrix markers after in vitro incubation of rat liver lysosomes with various polypeptides. The addition of basic polypeptides, polylysine or protamine, to the suspension of lysosomes brought about a profound alteration of lysosomal membrane, causing extensive leakage of lysosomal matrix enzymes. Electron microscopic observation revealed a remarkable aggregation of lysosomes by the basic polypeptides. Polyglutamic acid, an acidic polypeptide, did not produce such effect. ATP was found to stabilize lysosomes during incubation, particularly with basic polypeptides.
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
December 1969,
The Biochemical journal,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
January 2001,
Journal of inorganic biochemistry,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
December 1974,
Chemistry and physics of lipids,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
April 1970,
Biochemistry,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
April 1974,
Journal of biochemistry,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
May 1979,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
November 1967,
Biochimica et biophysica acta,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
September 1982,
FEBS letters,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
June 1996,
Pharmaceutical research,
E V Rukavishnikova, and
T A Korolenko, and
T Sassa, and
T Oka, and
S Horiuchi, and
Y Natori
April 2016,
Cold Spring Harbor protocols,
