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A new two-step centrifugation technique is described for the rapid preparation of viable pure polymorphonuclear granulocytes from 9.5 ml of venous blood. The phagocytosis of these cells was stimulated by soluble DNA-anti-DNA complexes, produced in vitro by incubating high mol. wt. DNA with sera obtained from patients with severe SLE. The measure of phagocytosis was the amount of nitroblue-tetrazolium (NBT), reduced by the active microphages. The reduced NBT was estimated by counting the formazane positive cells or by extracting and measuring it spectrophotometrically. The immune-complex phagocytosis of control human granulocytes showed a linear relationship with the cell count per reaction mixture and displayed a bell-shaped dose-response curve as function of the immune-complex. The immune-complex phagocytosis by SLE granulocytes was decreased by about 40 per cent as compared to normal granulocytes.
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
January 1980,
Acta medica Academiae Scientiarum Hungaricae,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
October 1978,
Laboratory investigation; a journal of technical methods and pathology,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
December 1969,
The Japanese journal of experimental medicine,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
January 1995,
Schweizer Monatsschrift fur Zahnmedizin = Revue mensuelle suisse d'odonto-stomatologie = Rivista mensile svizzera di odontologia e stomatologia,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
February 1979,
Annals of the rheumatic diseases,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
January 1991,
Acta morphologica Hungarica,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
May 1974,
The New England journal of medicine,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
February 1985,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
August 1981,
The Journal of protozoology,
L Molnár, and
J M Baló-Banga, and
A Horváth, and
J Leibinger, and
E Ablonczy, and
M Nováki
January 1994,
Methods in enzymology,
