Biomaterial Database

[Penicillin acylase from Escherichia coli: catalytically active subunits]. 1995

V E Kabakov, and N L Kliachko, and A V Levashov

Gel filtration under denaturing conditions was used to isolate the alpha- and beta-subunits of penicillin acylase (PA). Refolded subunits were obtained through removing urea by dialysis. Both renatured subunits were catalytically active during hydrolysis of phenylacetic acid p-nitroanilide; this activity decreased after addition of a serine-specific inhibitor--phenylmethanesulfonyl fluoride. The subunits were also active in reversed micelles of Aerosol OT (AOT) in octane, the optimum hydration degree being 11.9 and 17.5 for the light (alpha) and heavy (beta) subunits, respectively. The positions of the maxima were consistent with both theoretically calculated optimum hydration degrees and the earlier reported profile of enzymatic activity for native PA in reversed micelles.

UI	MeSH Term	Description	Entries
D008823	Micelles	Particles consisting of aggregates of molecules held loosely together by secondary bonds. The surface of micelles are usually comprised of amphiphatic compounds that are oriented in a way that minimizes the energy of interaction between the micelle and its environment. Liquids that contain large numbers of suspended micelles are referred to as EMULSIONS.	Micelle
D010399	Penicillin Amidase	An enzyme catalyzing the hydrolysis of penicillin to penicin and a carboxylic acid anion. EC 3.5.1.11.	Penicillin Acylase,Penicillin Amidohydrolase,Benzylpenicillin Acylase,Cephalosporin Acylase,Penicillin G Acylase,Penicillin V Acylase,Acylase, Benzylpenicillin,Acylase, Cephalosporin,Acylase, Penicillin,Acylase, Penicillin G,Acylase, Penicillin V,Amidase, Penicillin,Amidohydrolase, Penicillin
D010664	Phenylmethylsulfonyl Fluoride	An enzyme inhibitor that inactivates IRC-50 arvin, subtilisin, and the fatty acid synthetase complex.	Benzenemethanesulfonyl Fluoride,Phenylmethanesulfonyl Fluoride,Fluoride, Benzenemethanesulfonyl,Fluoride, Phenylmethanesulfonyl,Fluoride, Phenylmethylsulfonyl
D002384	Catalysis	The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.	Catalyses
D002850	Chromatography, Gel	Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.	Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D004143	Dioctyl Sulfosuccinic Acid	All-purpose surfactant, wetting agent, and solubilizer used in the drug, cosmetics, and food industries. It has also been used in laxatives and as cerumenolytics. It is usually administered as either the calcium, potassium, or sodium salt.	Dioctyl Sulfosuccinates,Docusate,Aerosol OT,Colace,DEH-Na-SS,DOSS,Diethylhexyl Sodium Sulfosuccinate,Dioctyl Sulfosuccinate,Dioctyl Sulfosuccinic Acid, Ammonium Salt,Dioctyl Sulfosuccinic Acid, Barium Salt,Dioctyl Sulfosuccinic Acid, Calcium Salt,Dioctyl Sulfosuccinic Acid, Magnesium Salt,Dioctyl Sulfosuccinic Acid, Potassium Salt,Dioctyl Sulfosuccinic Acid, Sodium Salt,Dioctylsulfosuccinate,Docusate Calcium,Docusate Potassium,Docusate Sodium,Sodium Bis(2-ethylhexyl)sulfosuccinate,Sodium Dioctyl Sulfosuccinate,Sodium Dioctylsulphosuccinate,Sulfosuccinic Acid bis(2-Ethylhexyl) Ester,DEH Na SS,Dioctyl Sulfosuccinate, Sodium,Dioctylsulphosuccinate, Sodium,Sodium Sulfosuccinate, Diethylhexyl,Sulfosuccinate, Diethylhexyl Sodium,Sulfosuccinate, Dioctyl,Sulfosuccinates, Dioctyl
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006868	Hydrolysis	The process of cleaving a chemical compound by the addition of a molecule of water.
D000814	Aniline Compounds	Compounds that include the aminobenzene structure.	Phenylamine,Phenylamines,Anilines,Compounds, Aniline
D017510	Protein Folding	Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.	Protein Folding, Globular,Folding, Globular Protein,Folding, Protein,Foldings, Globular Protein,Foldings, Protein,Globular Protein Folding,Globular Protein Foldings,Protein Foldings,Protein Foldings, Globular