BIOMAT Database Logo BIOMAT Database Logo

Kinetics of metabolism in human kidney transplants measured by dynamic 31P NMR spectroscopy. 1995

H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Institut für Physikalische Chemie, Westfälische Wilhelms-Universität Münster, Bundesrepublik Deutschland.

The technique of ex vivo 31P NMR spectroscopy has been used for the investigation of metabolic turnover in 15 cadaveric human kidneys obtained from brain-dead donors for transplantation. Measurements were carried out at 1.5 T time-dependently during regular hypothermic storage in histidine-tryptophan-alpha-ketoglutarate solution. The minimum delay between explanation and spectroscopy was 2 h 41 min. In one case of a discarded organ the measurements could be extended over a period of 161 h 35 min. A detailed kinetic model describing monoexponential ischemic phosphate turnover, which accounts for various interrelations of the NMR visible metabolites, has been derived. Averaged velocity constants of the decays of nucleotides and phosphomono- and -diesters ranged from 0.0047 to 0.294h-1 at approximately 4 degrees C. Intracellular pH decreased monoexponentially with an averaged velocity constant of 0.31 h-1.

UI MeSH Term Description Entries
D007511 Ischemia A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION. Ischemias
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008433 Mathematics The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Mathematic
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D009682 Magnetic Resonance Spectroscopy Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING). In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D001926 Brain Death A state of prolonged irreversible cessation of all brain activity, including lower brain stem function with the complete absence of voluntary movements, responses to stimuli, brain stem reflexes, and spontaneous respirations. Reversible conditions which mimic this clinical state (e.g., sedative overdose, hypothermia, etc.) are excluded prior to making the determination of brain death. (From Adams et al., Principles of Neurology, 6th ed, pp348-9) Brain Dead,Coma Depasse,Irreversible Coma,Brain Deads,Coma, Irreversible,Death, Brain
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006859 Hydrogen The first chemical element in the periodic table with atomic symbol H, and atomic number 1. Protium (atomic weight 1) is by far the most common hydrogen isotope. Hydrogen also exists as the stable isotope DEUTERIUM (atomic weight 2) and the radioactive isotope TRITIUM (atomic weight 3). Hydrogen forms into a diatomic molecule at room temperature and appears as a highly flammable colorless and odorless gas. Protium,Hydrogen-1
D014019 Tissue Donors Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients. Organ Donors,Donors,Ovum Donors,Semen Donors,Transplant Donors,Donor,Donor, Organ,Donor, Ovum,Donor, Semen,Donor, Tissue,Donor, Transplant,Donors, Organ,Donors, Ovum,Donors, Semen,Donors, Tissue,Donors, Transplant,Organ Donor,Ovum Donor,Semen Donor,Tissue Donor,Transplant Donor

Related Publications

H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Energy metabolism measured by 31P magnetic resonance spectroscopy in the healthy human brain.
September 2022, Journal of neuroradiology = Journal de neuroradiologie,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Phospholipid metabolism in cancer cells monitored by 31P NMR spectroscopy.
November 1987, The Journal of biological chemistry,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
(ECTO) nucleotidase kinetics observed by 31P-NMR spectroscopy: resolution of signals.
November 1997, Pharmacological research,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
[Study of erythrocyte metabolism using 31P NMR-spectroscopy].
September 1986, Bratislavske lekarske listy,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Ribosome-Amplified Metabolism, RAMBO, Measured by NMR Spectroscopy.
June 2021, Biochemistry,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Improved technique for investigation of cell metabolism by 31P NMR spectroscopy.
February 1981, Bioscience reports,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Reproducibility of human cardiac 31P-NMR spectroscopy.
August 1996, NMR in biomedicine,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Human muscle energetics during voluntary and electrically induced isometric contractions as measured by 31P NMR spectroscopy.
July 1999, International journal of sports medicine,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
The metabolic state of the rat liver in vivo measured by 31P-NMR spectroscopy.
January 1986, Biochimica et biophysica acta,
H E Möller, and A Gaupp, and T Vestring, and K H Dietl, and P Vermathen, and B Buchholz, and P E Peters
Effect of photic stimulation on energy metabolism in the human brain measured by 31P-MR spectroscopy.
January 1996, The Journal of neuropsychiatry and clinical neurosciences,
Copied contents to your clipboard!