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A solid-phase enzyme immunoassay (ELISA) for measuring tetanus antitoxin activity in human serum is described; the assay is based on a combination of the indirect method and ultramicro analysis. This rapid test, which has the capacity to analyze 78 blood samples per reagent plate (at a volume of 10 microL of diluted serum per sample), is proposed as an alternative to the traditional mouse bioassay system based on the neutralization of a known dose of tetanus toxin. Results from both tests showed a high correlation in the lineal regression analysis (r = 0.99; CI95%: 0.985 to 0.993). It is recommended that the ultramicro ELISA assay be used in the field to evaluate tetanus toxoid vaccines and to identify hyperimmune plasmas suitable for producing antitetanus immunoglobulin.
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
January 1974,
Journal of postgraduate medicine,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
May 1984,
Ceskoslovenska epidemiologie, mikrobiologie, imunologie,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
March 1962,
Annals of allergy,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
April 1987,
Journal of biological standardization,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
April 1963,
British medical journal,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
January 1968,
British medical journal,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
January 1967,
New York state journal of medicine,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
September 1966,
Canadian Medical Association journal,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
September 1966,
Canadian Medical Association journal,
E M Fajardo, and
J L Fernández, and
R L Solís, and
B Portuondo, and
L Heredia, and
M Noroña, and
H Urquiza, and
M Amat
April 1950,
Wiener medizinische Wochenschrift (1946),
