-transparent.png){kind=logo}
Sphingosine is known to regulate a variety of cellular functions through protein kinase C-dependent or independent pathways. In an attempt to investigate differential functions of diacylglycerol kinase (DGK) isozymes, we tested the effect of sphingosine on DGK operating in intact Jurkat cells, a human T-cell line. We found that phosphatidic acid (PA) synthesized from endogenous diacylglycerol (DG) and exogenously added short-chain DGs like dioctanoylglycerol were markedly enhanced by approx. 20 microM sphingosine. Further studies such as the use of protein kinase C down-regulated cells, mass measurements of cellular DGs, analysis of molecular species of PA and the effect of exogenous DG on the conversion of endogenous DG to PA suggested that sphingosine directly activated cellular DGK having a broad specificity toward DG molecular species.
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
February 1989,
FEBS letters,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
February 1992,
Cellular immunology,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
November 1991,
Immunology letters,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
October 2006,
Ai zheng = Aizheng = Chinese journal of cancer,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
January 1998,
Advances in experimental medicine and biology,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
August 2003,
Assay and drug development technologies,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
June 1999,
Investigative ophthalmology & visual science,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
July 2000,
Electrophoresis,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
May 2018,
Cell biology international,
K Yamada, and
F Sakane, and
S Imai, and
H Takemura
April 1997,
European journal of immunology,
