The establishment of associative interrelations between nodule bacteria and cells of a callus culture of alfalfa roots, evaluated according to the criterion of nitrogenase activity, was accompanied by considerable inhibition of the plant cells in the period of maximum infection as well as by the dispersal of actively multiplying nodule bacteria on the surface of the callus cells in the first 7 days after infection. The dynamics of the localization of nodule bacteria on the surface of callus cells was studied by the method of scanning electron microscopy.