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Hexokinase isoenzyme composition has been noted to vary in different tissues and with the developmental and metabolic status of the cell. Until now these investigations were performed either by isoenzyme electrophoresis or by column chromatography. In this report we described an RNA-PCR method to evaluate the percentage of HK-1 and HK-2 in different rat tissues. Furthermore we applied the method to determine if a shift in isoform composition is detectable in human renal carcinomas compared to normal kidney tissue. In all of our specimens we were able to detect a shift toward HK-2 in the carcinoma specimens. We discuss a possible role for the detection of the shift in isoenzyme composition as a possible marker to discriminate between normal and malignant specimens.
V Adams, and
W Kempf, and
S Hassam, and
J Briner
August 2004,
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
July 1973,
Archives of biochemistry and biophysics,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
January 1999,
Anticancer research,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
July 1978,
Journal of the National Cancer Institute,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
October 2008,
Journal of clinical microbiology,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
November 1999,
Molecular genetics and metabolism,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
June 1982,
Molecular and cellular biochemistry,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
September 1972,
Doklady Akademii nauk SSSR,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
July 1998,
American journal of respiratory cell and molecular biology,
V Adams, and
W Kempf, and
S Hassam, and
J Briner
December 1997,
Molecular and cellular biochemistry,
