-transparent.png){kind=logo}
Recent studies have implicated leukemia inhibitory factor (LIF) in human joint disease. LIF is produced by cultured synovial cells and articular chondrocytes, stimulates cartilage and bone resorption, and has been detected in inflammatory exudates from arthritic joints. The aim of this study was to evaluate the effect of intraarticular injections of human recombinant LIF in the goat. Endotoxin-free, sterile normal saline containing 1 micrograms recombinant human LIF (rhLIF) was injected into the right radiocarpal joints (RCJs) of eight angora goats. The left RCJs were injected with an equivalent volume of vehicle alone (n = 6) or vehicle containing 1 micrograms human albumin (n = 2). Goat joints were examined for clinical features of inflammation, and synovial fluid (SF) was aspirated on days 0, 2, and 6 postinjection. Leukocyte counts and concentrations of keratan sulfate, IL-1 beta, and TNF-alpha were determined in the SF. Proteoglycan synthesis was determined ex vivo in cartilage explants obtained on day 6 postinjection. A statistically significant increase in joint swelling and effusion volume was observed in LIF-injected joints but not in control joints. In the LIF-injected RCJs, the leukocyte count increased from 82 +/- 9 cells/microliters before injection to 10,300 +/- 3357 cells/microliters at day 2 postinjection (p < 0.005) and declined to 678 +/- 113 cells/microliters at day 6 postinjection. Polymorphonuclear leukocytes and monocyte/macrophages predominated in the infiltrate. No appreciable change in leukocyte counts was observed in control joints.(ABSTRACT TRUNCATED AT 250 WORDS)
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
November 1986,
Proceedings of the National Academy of Sciences of the United States of America,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
March 1976,
The Journal of clinical investigation,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
August 1992,
Arthritis and rheumatism,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
August 1984,
Biochimica et biophysica acta,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
November 1978,
Biochimica et biophysica acta,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
June 1975,
Annals of the New York Academy of Sciences,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
May 1977,
Arthritis and rheumatism,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
February 1991,
The Journal of rheumatology. Supplement,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
August 1994,
Proceedings of the National Academy of Sciences of the United States of America,
G J Carroll, and
M C Bell, and
H M Chapman, and
J N Mills, and
W F Robinson
September 1988,
British journal of pharmacology,
