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Fetal antigen 2 (FA-2) has been identified in amniotic fluid and shown to be of fetal origin. In this study we have extended previous observations on FA-2 heterogeneity with respect to both size and charge using gel filtration, ion-exchange chromatography, non-dissociating polyacrylamide gel electrophoresis and sodium dodecyl sulphate polyacrylamide gel electrophoresis. From the diversity of forms we have been able to define two principal FA-2 types, type A and type B. Type A has a high molecular mass (140 kDa), has subunits of 33 kDa and 29 kDa, and elutes at approximately 0.27 mol/l sodium chloride from diethylaminoethyl (DEAE)-Sephacel. Type B has the same mass and subunits as type A, but elutes at approximately 0.24 mol/l sodium chloride from DEAE-Sephacel. Other low molecular mass forms of FA-2 have also been identified. All FA-2 forms described were shown to be common to all amniotic fluid samples studied and were not attributable to artefacts of collection or storage. It was also demonstrated that the recently described FA-2 RIA is specific for FA-2 types A and B and the conversion of arbitrary units FA-2 into micrograms applies to type A. The typing is discussed with respect to (i) the aminopropeptide of the alpha 1 chain of human procollagen type I, (ii) the 24 kDa phosphoprotein in developing bone and (iii) fetal calf ligament protein 1 (FCL-1), suggesting that they are the same protein.
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
March 1983,
American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
July 1986,
American journal of obstetrics and gynecology,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
February 1983,
Clinical genetics,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
January 1978,
Biology of the neonate,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
January 1980,
Annales de genetique,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
January 1986,
Prenatal diagnosis,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
July 1976,
Biomedicine / [publiee pour l'A.A.I.C.I.G.],
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
March 1978,
The American review of respiratory disease,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
April 1982,
Prenatal diagnosis,
K M Price, and
R Silman, and
P Armstrong, and
B Teisner, and
J G Grudzinskas
January 1980,
Clinical endocrinology,
