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We succeeded in the isolation of osteocytes from parietal bones of 16-day-old chick embryos. Isolated osteocytes showed a typical stellate morphology. More than 95% of these cells reacted with the osteocyte-specific antibody OB 7.3. In culture osteocytes formed gap junctions with each other, as could be established by ACAS. Sixteen-millimeter time-lapse microcinematography of the cells also demonstrated the formation of intercellular connections and gap junctions, and portrayed the interaction between osteocytes and osteoclasts: osteocytes seemed to inhibit osteoclast activity. This cinematography also showed the ability of osteocytes to proliferate after they had been disconnected from each other. Thereafter these cells redifferentiated into osteoblasts that became embedded in bone matrix produced by themselves. These findings suggest that osteocytes might be involved in bone formation during remodeling.
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
February 1973,
Experientia,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
August 1973,
Experimental cell research,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
August 2010,
Journal of visualized experiments : JoVE,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
March 1962,
Laboratory investigation; a journal of technical methods and pathology,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
January 1985,
Revista espanola de oncologia,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
January 1974,
Ontogenez,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
March 1974,
Microscopica acta,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
January 1967,
Acta biologica Academiae Scientiarum Hungaricae,
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
November 1978,
Journal of immunology (Baltimore, Md. : 1950),
K Tanaka, and
T Matsuo, and
M Ohta, and
T Sato, and
K Tezuka, and
P J Nijweide, and
Y Katoh, and
Y Hakeda, and
M Kumegawa
January 1976,
Proceedings of the Royal Society of London. Series B, Biological sciences,
