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Kinetics of the reaction of baker's yeast glucose-6-phosphate dehydrogenase with 5,5'-dithiobis(2-nitrobenzoic acid). 1995

S A Adediran, and M R Gbadegesin
Department of Chemistry, University of Ilorin, Nigeria.

The kinetics of the reaction of baker's yeast glucose-6-phosphate dehydrogenase with excess 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) were studied at pH 8.5 and 30 degrees C and at constant ionic strength of 0.01 and in the absence and in the presence of NADP+ or glucose 6-phosphate. The reaction follows pseudo-first-order kinetics irrespective of whether these substrates are absent or present. The observed pseudo-first-order rate constant is reduced in the presence of NADP+ or glucose 6-phosphate but on a molar basis, glucose 6-phosphate is more effective than NADP+ in protecting the sulfhydryl groups of the enzyme against the reaction with DTNB. In the presence of NADP+, the observed pseudo-first-order rate constant decreases to a constant, but finite, value at a saturating coenzyme concentration. The effect of NADP+ on the rate constant is consistent with the presence of noninteracting coenzyme binding sites in baker's yeast glucose-6-phosphate dehydrogenase.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D009249 NADP Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed) Coenzyme II,Nicotinamide-Adenine Dinucleotide Phosphate,Triphosphopyridine Nucleotide,NADPH,Dinucleotide Phosphate, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide Phosphate,Nucleotide, Triphosphopyridine,Phosphate, Nicotinamide-Adenine Dinucleotide
D004228 Dithionitrobenzoic Acid A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate. 5,5'-Dithiobis(2-nitrobenzoic Acid),DTNB,Ellman's Reagent,5,5'-Dithiobis(nitrobenzoate),Acid, Dithionitrobenzoic,Ellman Reagent,Ellmans Reagent,Reagent, Ellman's
D005954 Glucosephosphate Dehydrogenase Glucose-6-Phosphate Dehydrogenase,Dehydrogenase, Glucose-6-Phosphate,Dehydrogenase, Glucosephosphate,Glucose 6 Phosphate Dehydrogenase
D005958 Glucosephosphates
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D019298 Glucose-6-Phosphate An ester of glucose with phosphoric acid, made in the course of glucose metabolism by mammalian and other cells. It is a normal constituent of resting muscle and probably is in constant equilibrium with fructose-6-phosphate. (Stedman, 26th ed) Glucose 6 Phosphate

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