Biomaterial Database

Finding of a homarine-synthesizing enzyme in turban shell and some properties of the enzyme. 1995

H Nishitani, and S Kikuchi, and K Okumura, and H Taguchi

R & D Center, Terumo Corporation, Kanagawa, Japan.

A homarine-synthesizing enzyme was found for the first time in cell-free extract from turban shell (Batillus cornutus) and the enzyme was purified 36.2-fold and characterized. Properties of the enzyme were as follows: substrates were picolinic acid (pyridine-2-carboxylic acid) and S-adenosyl-L-methionine; optimum temperature for the enzymic reaction was 25 degrees C; optimum pH for the enzymic reaction was 6.3; and the Km values for picolinic acid and S-adenosyl-L-methionine were calculated at 317 and 14.5 microM, respectively. Among pyridine carboxylic acids, only picolinic acid was methylated with S-adenosyl-L-methionine by this enzyme. The molecular weight of the enzyme was estimated to be 70,800. The enzyme activity was inhibited by heavy metal ions, S-adenosyl-L-homocysteine, adenosine, homocysteine, and sinefungin. Homarine, which is an osmotic pressure regulator, morphogen, etc.; is enzymatically synthesized by the methylation of picolinic acid with S-adenosyl-L-methionine and the enzyme activity may be controlled by S-adenosyl-L-homocysteine (reaction product) and its related compounds.

UI	MeSH Term	Description	Entries
D007448	Invertebrates	Animals that have no spinal column.	Brachiopoda,Mesozoa,Brachiopodas,Invertebrate,Mesozoas
D008780	Methyltransferases	A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1.	Methyltransferase
D010848	Picolinic Acids	Compounds with general formula C5H4N(CO2H) derived from PYRIDINE, having a carboxylic acid substituent at the 2-position.	Acids, Picolinic
D002413	Cations, Divalent	Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.	Divalent Cations
D004791	Enzyme Inhibitors	Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.	Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D005998	Glycine	A non-essential amino acid. It is found primarily in gelatin and silk fibroin and used therapeutically as a nutrient. It is also a fast inhibitory neurotransmitter.	Aminoacetic Acid,Glycine, Monopotassium Salt,Glycine Carbonate (1:1), Monosodium Salt,Glycine Carbonate (2:1), Monolithium Salt,Glycine Carbonate (2:1), Monopotassium Salt,Glycine Carbonate (2:1), Monosodium Salt,Glycine Hydrochloride,Glycine Hydrochloride (2:1),Glycine Phosphate,Glycine Phosphate (1:1),Glycine Sulfate (3:1),Glycine, Calcium Salt,Glycine, Calcium Salt (2:1),Glycine, Cobalt Salt,Glycine, Copper Salt,Glycine, Monoammonium Salt,Glycine, Monosodium Salt,Glycine, Sodium Hydrogen Carbonate,Acid, Aminoacetic,Calcium Salt Glycine,Cobalt Salt Glycine,Copper Salt Glycine,Hydrochloride, Glycine,Monoammonium Salt Glycine,Monopotassium Salt Glycine,Monosodium Salt Glycine,Phosphate, Glycine,Salt Glycine, Monoammonium,Salt Glycine, Monopotassium,Salt Glycine, Monosodium
D000241	Adenosine	A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.	Adenocard,Adenoscan
D000470	Alkaloids	Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)	Alkaloid,Plant Alkaloid,Plant Alkaloids,Alkaloid, Plant,Alkaloids, Plant
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012435	S-Adenosylhomocysteine	5'-S-(3-Amino-3-carboxypropyl)-5'-thioadenosine. Formed from S-adenosylmethionine after transmethylation reactions.	S Adenosylhomocysteine,Adenosylhomocysteine, S