Biomaterial Database

Room temperature ESR spectra of Rhus vernicifera laccase and derivatives. 1995

T Sakurai, and J Takahashi

College of Liberal Arts and Sciences, Kanazawa University, Ishikawa, Japan.

Although both the type 1 and type 2 coppers of Rhus vernicifera laccase are fully ESR detectable at 77 K, only 30% of the type 2 copper are in the cupric form at room temperature. The residual 70% of the type 2 copper was easily transformed into the ESR detectable form by irradiating the resting enzyme with microwave of 200 mW. The enzyme activity did not change by the irradiation with high-powered microwave, indicating that the type 2 copper can be in both the ESR detectable and ESR undetectable forms in solution. The room temperature ESR spectra of the type 2 copper-depleted laccase and of the azide-bound type 3 copper signals were also measured at room temperature and compared with those at 77 K.

UI	MeSH Term	Description	Entries
D010088	Oxidoreductases	The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)	Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D010947	Plants, Toxic	Plants or plant parts which are harmful to man or other animals.	Plants, Poisonous,Plant, Poisonous,Plant, Toxic,Poisonous Plant,Poisonous Plants,Toxic Plant,Toxic Plants
D003300	Copper	A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.	Copper-63,Copper 63
D004578	Electron Spin Resonance Spectroscopy	A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.	ENDOR,Electron Nuclear Double Resonance,Electron Paramagnetic Resonance,Paramagnetic Resonance,Electron Spin Resonance,Paramagnetic Resonance, Electron,Resonance, Electron Paramagnetic,Resonance, Electron Spin,Resonance, Paramagnetic
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D012251	Toxicodendron	A genus (formerly part of Rhus genus) of shrubs, vines, or trees that yields a highly allergenic oleoresin which causes a severe contact dermatitis (DERMATITIS, TOXICODENDRON). The most toxic species are Toxicodendron vernix (poison sumac), T. diversilobum (poison oak), and T. radicans (poison ivy). T. vernicifera yields a useful varnish from which certain enzymes (laccases) are obtained.	Ivy, Poison,Oak, Poison,Poison Ivy,Poison Oak,Poison Sumac,Sumac, Poison,Rhus toxicodendron,Ivies, Poison,Oaks, Poison,Poison Ivies,Poison Oaks,Poison Sumacs,Rhus toxicodendrons,Sumacs, Poison,Toxicodendrons,toxicodendron, Rhus
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D042845	Laccase	A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.	Laccase B,Laccase I,Laccase II,Laccase III,Urishiol Oxidase,p-Diphenol Oxidase,Oxidase, Urishiol,Oxidase, p-Diphenol,p Diphenol Oxidase