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The analysis of haemoglobin is routine in medical laboratories for the purpose of assessing blood disorders and related pathologies. Haemoglobin is structurally diverse and possesses many variant forms, some disease-producing. With recent improvements in technology, capillary electrophoresis is now being adapted in the clinical laboratory. This paper describes the analysis of haemoglobin variants using uncoated fused-silica capillaries. The effects of using different buffer salts at different concentrations with different buffer pH values to separate haemoglobin variants in these capillaries are described.
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