BIOMAT Database Logo BIOMAT Database Logo

Isolation and photodynamic effects of hematoporphyrin derivative components: a chromatographic analysis of the starting materials. 1995

J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
Health Research Center, Southern University, Baton Rouge, LA 70813, USA.

Twenty different fractions of hematoporphyrin derivatives (HpD) and eight fractions of an HpD dimer mixture were isolated utilizing isocratic reversed-phase ion-pair high-performance liquid chromatography. These fractions were characterized by UV-visible and fluorescence spectrophotometry. Fluorescence quantum yields and photokill efficiency for each fraction in PTK2 epithelial cells were obtained. Results indicate that some part of the photoactivity exhibited by HpD may be due to impurities present in the HpD starting material, hematoporphyrin-IX dihydrochloride, depending on its source. It was also found that hematoporphyrin D, a commercial acetylated product formed during synthesis of HpD, contained a higher percentage of monomers than would be expected.

UI MeSH Term Description Entries
D007614 Macropodidae A family of herbivorous leaping MAMMALS of Australia, New Guinea, and adjacent islands. Members include kangaroos, wallabies, quokkas, and wallaroos. Kangaroos,Macropus,Petrogale,Quokkas,Setonix,Wallabies,Wallabies, Rock,Wallaroo,Macropus robustus,Kangaroo,Petrogales,Quokka,Rock Wallabies,Rock Wallaby,Wallaby,Wallaby, Rock,Wallaroos
D010778 Photochemotherapy Therapy using oral or topical photosensitizing agents with subsequent exposure to light. Blue Light Photodynamic Therapy,Photodynamic Therapy,Red Light PDT,Red Light Photodynamic Therapy,Therapy, Photodynamic,Light PDT, Red,PDT, Red Light,Photochemotherapies,Photodynamic Therapies,Therapies, Photodynamic
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000970 Antineoplastic Agents Substances that inhibit or prevent the proliferation of NEOPLASMS. Anticancer Agent,Antineoplastic,Antineoplastic Agent,Antineoplastic Drug,Antitumor Agent,Antitumor Drug,Cancer Chemotherapy Agent,Cancer Chemotherapy Drug,Anticancer Agents,Antineoplastic Drugs,Antineoplastics,Antitumor Agents,Antitumor Drugs,Cancer Chemotherapy Agents,Cancer Chemotherapy Drugs,Chemotherapeutic Anticancer Agents,Chemotherapeutic Anticancer Drug,Agent, Anticancer,Agent, Antineoplastic,Agent, Antitumor,Agent, Cancer Chemotherapy,Agents, Anticancer,Agents, Antineoplastic,Agents, Antitumor,Agents, Cancer Chemotherapy,Agents, Chemotherapeutic Anticancer,Chemotherapy Agent, Cancer,Chemotherapy Agents, Cancer,Chemotherapy Drug, Cancer,Chemotherapy Drugs, Cancer,Drug, Antineoplastic,Drug, Antitumor,Drug, Cancer Chemotherapy,Drug, Chemotherapeutic Anticancer,Drugs, Antineoplastic,Drugs, Antitumor,Drugs, Cancer Chemotherapy
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry
D017319 Photosensitizing Agents Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue. These compounds can be administered topically or systemically and have been used therapeutically to treat psoriasis and various types of neoplasms. Photosensitizer,Photosensitizers,Photosensitizing Agent,Photosensitizing Effect,Photosensitizing Effects,Agent, Photosensitizing,Agents, Photosensitizing,Effect, Photosensitizing,Effects, Photosensitizing

Related Publications

J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
[Microcirculatory effects of photodynamic therapy with hematoporphyrin derivative].
February 1991, Laryngo- rhino- otologie,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
[Chromatographic analysis of a hematoporphyrin derivative and its active fractions].
September 1984, Yao xue xue bao = Acta pharmaceutica Sinica,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
The photodynamic properties of a particular hematoporphyrin derivative.
October 1960, Archives of dermatology,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
Effects of hematoporphyrin derivative and photodynamic therapy on atherosclerotic rabbits.
October 1985, The American journal of cardiology,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
Microemulsion and micellar electrokinetic chromatography of Hematoporphyrin D: a starting material of hematoporphyrin derivative.
February 2005, Electrophoresis,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
The main photosensitizing components of hematoporphyrin derivative.
February 1982, Cancer letters,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
Comparison of fluorescence and photodynamic activities of whole hematoporphyrin derivative and its enriched active components.
December 1985, Journal of the National Cancer Institute,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
Wavelength dependence of hematoporphyrin derivative photodynamic treatment effects on rat ears.
September 1990, Photochemistry and photobiology,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
Photodynamic effects on cells in vitro exposed to hematoporphyrin derivative and light.
June 1983, Photochemistry and photobiology,
J W Owens, and L Yang, and G Adeola, and M Robins, and R Smith, and R Robinson, and N Elayan, and L McMahon
Photodynamic therapy of tumors: effects of hematoporphyrin derivative on normal rat intestine.
November 1994, Cellular and molecular biology (Noisy-le-Grand, France),
Copied contents to your clipboard!