-transparent.png){kind=logo}
Genes encoding beta-ketothiolase (phaA), acetoacetyl-CoA reductase (phaB) and PHB-synthase (phaC) from R. meliloti 41, together with a fourth gene, referred to as ORF1, presumed to be involved in PHB biosynthesis, have been cloned and sequenced. phaA, phaB and ORF1 were identified by heterologous hybridization on a cosmid library, while phaC was isolated by cloning the transposon-tagged fragment from a R. meliloti PHB- Tn5 mutant. phaA and phaB were functionally expressed in Escherichia coli while phaC was able to complement a PHB- strain of R. meliloti 41. The three genes were sufficient to direct the production of polyhydroxyalkanoate in E. coli. The homology of ORF1 with an ORF located near the PHB genes in two phototrophic bacteria suggests its involvement in PHB synthesis.
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
January 1997,
Applied and environmental microbiology,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
January 1976,
Seikagaku. The Journal of Japanese Biochemical Society,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
March 1987,
Biomaterials,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
June 1998,
Canadian journal of microbiology,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
January 1983,
Molecular & general genetics : MGG,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
February 2007,
Microbiology (Reading, England),
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
February 1999,
Journal of bacteriology,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
May 1982,
Journal of bacteriology,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
November 2007,
Applied microbiology and biotechnology,
R Tombolini, and
S Povolo, and
A Buson, and
A Squartini, and
M P Nuti
September 1989,
The Journal of biological chemistry,
