Biomaterial Database

All-or-none augmentation of Ca2+ sensitivity in alpha-toxin-permeabilized single smooth muscle cells from guinea-pig taenia caecum. 1995

M Mita, and T Hashimoto

Department of Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.

1. Isolated smooth muscle cells from guinea-pig taenia caecum were permeabilized by use of Staphylococcus aureus alpha-toxin, and the sarcoplasmic reticulum Ca2+ store was depleted by exposure to 0.1 microM A23187. 2. Shortening of alpha-toxin-permeabilized single smooth muscle cells was induced by increasing free Ca2+ but was not induced by 0.2 microM free Ca2+. 3. Shortening of the permeabilized cells was caused by application of acetylcholine (ACh) with free Ca2+ concentration held at 0.2 microM. Permeabilized smooth muscle cells responded to 0.3 microM or 1 microM ACh with 0.2 microM Ca2+ with maximal shortening. The concentration-response relationship to ACh had a very steep slope and the cell shortening appeared to be an all-or-none response rather than a graded response, as was the shortening of intact cells to ACh. 4. The shortening of permeabilized cells was also induced by application of guanosine 5'-triphosphate (GTP) with 0.2 microM free Ca2+, showing an all-or-none response. The threshold concentration of GTP that induced an all-or-none response was between 10 microM and 30 microM. 5. These results suggest that Ca2+ sensitivity is augmented by stimulation of the muscarinic receptor or GTP-binding protein(s) in an all-or-none manner. It seems probable that this contributes to the all-or-none response to ACh in intact smooth muscle cells.

UI	MeSH Term	Description	Entries
D007476	Ionophores	Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.	Ionophore
D008858	Microscopy, Phase-Contrast	A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.	Phase-Contrast Microscopy,Microscopies, Phase-Contrast,Microscopy, Phase Contrast,Phase Contrast Microscopy,Phase-Contrast Microscopies
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009130	Muscle, Smooth	Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)	Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D011976	Receptors, Muscarinic	One of the two major classes of cholinergic receptors. Muscarinic receptors were originally defined by their preference for MUSCARINE over NICOTINE. There are several subtypes (usually M1, M2, M3....) that are characterized by their cellular actions, pharmacology, and molecular biology.	Muscarinic Acetylcholine Receptors,Muscarinic Receptors,Muscarinic Acetylcholine Receptor,Muscarinic Receptor,Acetylcholine Receptor, Muscarinic,Acetylcholine Receptors, Muscarinic,Receptor, Muscarinic,Receptor, Muscarinic Acetylcholine,Receptors, Muscarinic Acetylcholine
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002432	Cecum	The blind sac or outpouching area of the LARGE INTESTINE that is below the entrance of the SMALL INTESTINE. It has a worm-like extension, the vermiform APPENDIX.	Cecums
D002463	Cell Membrane Permeability	A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.	Permeability, Cell Membrane
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D006160	Guanosine Triphosphate	Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.	GTP,Triphosphate, Guanosine