BIOMAT Database Logo BIOMAT Database Logo

Crystallization and preliminary X-ray analysis of outer membrane phospholipase A from Escherichia coli. 1995

M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.

The outer membrane phospholipase A (OMPLA) of Escherichia coli is one of the few integral outer membrane proteins displaying enzymatic activity. It is encoded as a mature protein of 269 amino acids preceded by a signal sequence of 20 amino acids. There is no sequence homology with water-soluble lipases and phospholipases. Crystals of the mature enzyme were obtained at 22 degrees C from 24-28% (v/v) 2-methyl-2,4-pentanediol in Bis-Tris buffer, pH 5.9-6.0, with 1 mM calcium chloride and 1.5% (w/v) beta-octylglucoside. They have the symmetry of the trigonal spacegroup P3(1)21 (or P3(2)21) with cell dimensions of a = b = 79.6 A and c = 102.8 A (alpha = beta = 90 degrees, gamma = 120 degrees). Native crystals diffract to a resolution of 2.6 A.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010741 Phospholipases A Phospholipases that hydrolyze one of the acyl groups of phosphoglycerides or glycerophosphatidates.
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies

Related Publications

M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray analysis of phosphoporin from the outer membrane of Escherichia coli.
December 1991, Journal of molecular biology,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30.
March 2004, Acta crystallographica. Section D, Biological crystallography,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli.
December 2006, Acta crystallographica. Section F, Structural biology and crystallization communications,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Expression, crystallization and preliminary X-ray crystallographic studies of the outer membrane protein OmpW from Escherichia coli.
April 2006, Acta crystallographica. Section F, Structural biology and crystallization communications,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray analysis of FlhD from Escherichia coli.
November 1998, Journal of structural biology,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray analysis of Mlc from Escherichia coli.
February 2005, Acta crystallographica. Section F, Structural biology and crystallization communications,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray crystallographic analysis of YgjG from Escherichia coli.
September 2012, Acta crystallographica. Section F, Structural biology and crystallization communications,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray analysis of alpha-xylosidase from Escherichia coli.
February 2005, Acta crystallographica. Section F, Structural biology and crystallization communications,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray analysis of shikimate dehydrogenase from Escherichia coli.
April 2000, Acta crystallographica. Section D, Biological crystallography,
M Blaauw, and N Dekker, and H M Verheij, and K H Kalk, and B W Dijkstra
Crystallization and preliminary X-ray analysis of AzoR (azoreductase) from Escherichia coli.
April 2005, Acta crystallographica. Section F, Structural biology and crystallization communications,
Copied contents to your clipboard!