Biomaterial Database

A developmentally regulated member of the sialyltransferase family (ST8Sia II, STX) is a polysialic acid synthase. 1995

N Kojima, and Y Yoshida, and S Tsuji

Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.

We found polysialic acid synthase activity of ST8Sia II (STX) in vitro and in vivo. Previously, we showed that mouse ST8Sia II exhibits alpha 2,3-sialylated N-glycan alpha 2,8-sialyltransferase activity, but the polysialic acid synthase activity of ST8Sia II was not detected at that time [Kojima, N. et al. (1995) FEBS Lett. 360, 1-4]. When fetuin was [14C]sialylated with ST8Sia II and then its N-linked oligosaccharides were analyzed, a part of the N-linked oligosaccharides was eluted in the void volume from a Sephadex G-50 column, and was eluted in the void volume from a Sephadex G-50 column, and was eluted from the DEAE-Toyopearl column at almost the same salt concentration as that where colomic acid was eluted. In addition, a series of 14C-labeled oligo-sialic acids were obtained from the oligosaccharides on partial mild acid hydrolysis. These results indicated that a part of N-linked oligosaccharides of fetuin were polysialylated with ST8Sia II. Transfection of ST8Sia II gene into several cell lines including NIH3T3 led to the expression of polysialic acids on the cell surface. Thus, ST8Sia II can directly synthesize polysialic acid chains on alpha 2,3-sialylated N-linked oligosaccharides of glycoproteins without any initiator sialytransferase.

UI	MeSH Term	Description	Entries
D009844	Oligosaccharides	Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.	Oligosaccharide
D011994	Recombinant Proteins	Proteins prepared by recombinant DNA technology.	Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D000509	alpha-Fetoproteins	The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life.	alpha-Fetoprotein,alpha Fetoprotein,alpha Fetoproteins
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012794	Sialic Acids	A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.	N-Acetylneuraminic Acids,Acids, N-Acetylneuraminic,Acids, Sialic,N Acetylneuraminic Acids
D012799	Sialyltransferases	A group of enzymes with the general activity CMP-N-acetylneuraminate:acceptor N-acetylneuraminyl transferase. They catalyze the transfer of N-ACETYLNEURAMINIC ACID from CMP-N-ACETYLNEURAMINIC ACID to an acceptor, which is usually the terminal sugar residue of an oligosaccharide, a glycoprotein, or a glycolipid.	Glycoprotein Sialyltransferases,Glycosyltransferase Family 29,Sialyltransferase,Ectosialyltransferase,Glycoprotein Sialyltransferase,Sialyltransferase, Glycoprotein,Sialyltransferases, Glycoprotein
D014162	Transfection	The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.	Transfections
D014407	Tumor Cells, Cultured	Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.	Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D015971	Gene Expression Regulation, Enzymologic	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.	Enzymologic Gene Expression Regulation,Regulation of Gene Expression, Enzymologic,Regulation, Gene Expression, Enzymologic