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We describe a fully enzymic method for manual and continuous-flow colorimetric assay of triacylglycerols (triglycerides) in serum. Triglycerides are enzymically hydrolyzed in 10 min by lipase and microbial esterase. The resulting free glycerol is measured enzymically by glycerol kinase and glycerol-3-phosphate dehydrogenase. The NADH so formed is oxidized by coupling with a tetrazolium salt/diaphorase system. The test follows Beer's law to 8 g/L, and the final color is stable for at least 1 h for serum, 15 min for aqueous triolein standards. The manual assay requires only 25 microliter of serum and few manipulations. A specific triolein standard was developed for calibrating the manual method. For the continuous-flow method, calibration is made with four concentrations of glycerol standard. The procedure is sensitive, has good precision and accuracy, and gives results that compare well with chemical and enzymic commercial kit methods.
R E Megraw, and
D E Dunn, and
H G Biggs
March 1982,
Clinical chemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
July 1981,
Analytical biochemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
July 1951,
The Journal of physiology,
R E Megraw, and
D E Dunn, and
H G Biggs
September 1978,
Clinica chimica acta; international journal of clinical chemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
December 1973,
Clinical chemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
November 1981,
Clinical chemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
January 1980,
Clinical chemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
February 1984,
Clinical chemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
January 1977,
Annals of clinical biochemistry,
R E Megraw, and
D E Dunn, and
H G Biggs
April 1980,
Clinical chemistry,
