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Strains of Helicobacter pylori, the bacterium associated with gastritis, peptic ulcer disease, and gastric cancer in humans, express different degrees of hemolysis on agar containing erythrocytes (RBC). Here we report the isolation and characterization of six recombinant clones from a genomic library of H. pylori ATCC 49503 that confer on Escherichia coli the ability to lyse sheep RBC. DNA hybridizations indicated no sequence homology among these hemolytic clones. Hybridization mapping of them to an ordered H. pylori cosmid library identified their separate chromosomal locations. One clone hybridized to two regions separated by approximately 200 kb. The specificities of the hemolytic activities of these clones were tested with RBC from humans, monkeys, cattle, horses, guinea pigs, rabbits, and chickens as well as with RBC from sheep. One clone conferred the ability to lyse RBC from five species, a second clone allowed the lysis of RBC from four of these species, three other clones allowed the lysis of RBC from three of these species, and the sixth clone allowed the lysis of RBC from just two species. We propose that some or all of the genes that confer these various hemolytic activities contribute to pathogen-host tissue interactions and that the different specificities seen here are important for H. pylori infections of humans of different genotypes or disease states.
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
February 1998,
FEMS microbiology letters,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
December 2009,
The protein journal,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
February 2004,
Proceedings of the National Academy of Sciences of the United States of America,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
January 2007,
Life sciences,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
October 1992,
Molecular microbiology,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
April 2014,
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
October 1997,
Medical microbiology and immunology,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
December 2000,
Molecules and cells,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
October 1996,
The Biochemical journal,
E S Drazek, and
A Dubois, and
R K Holmes, and
D Kersulyte, and
N S Akopyants, and
D E Berg, and
R L Warren
July 2004,
Biochemical and biophysical research communications,
