Methicillin-resistant Staphylococcus aureus subtyping: interest of combined antibiotyping and esterase electrophoretic typing. 1995

N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
Laboratoire de Bactériologie, Hôpital Albert Chenevier, Créteil, France.

Ninety-four methicillin-resistant Staphylococcus aureus isolates (MRSA) were characterized by means of two typing methods, antibiotyping and esterase electrophoretic typing. Antibiotyping, recorded on the basis of susceptibility testing of 13 antimicrobial agents, allowed the description of 18 antibiotypes, four of which comprised 30, 14, 14 and 12 strains respectively. Esterase electrophoretic typing, based on esterase activity against seven synthetic substrates after polyacrylamide-agarose gel electrophoresis, led to the description of 12 electrophoretic types, two of which were predominant with 60 and 20 strains respectively. The combined use of both typing methods yielded 32 combinations, three of which were predominant with 21, 12 and 11 strains respectively. A good differentiation of strains was achieved, particularly when the antibiotype was correlated to the electrophoretic type. Thus, the combination of antibiotyping with esterase electrophoretic typing may be proposed as a well-suited method for the characterization of MRSA strains.

UI MeSH Term Description Entries
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D004950 Esterases Any member of the class of enzymes that catalyze the cleavage of an ester bond and result in the addition of water to the resulting molecules. Esterase
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D013211 Staphylococcus aureus Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D015373 Bacterial Typing Techniques Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping. Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin
D016106 Methicillin Resistance Non-susceptibility of a microbe to the action of METHICILLIN, a semi-synthetic penicillin derivative. Methicillin-Resistant,Methicillin Resistant,Resistance, Methicillin

Related Publications

N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
November 1987, Journal of medical microbiology,
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
July 1988, Journal of medical microbiology,
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
May 1999, Journal of medical microbiology,
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
November 1986, British medical journal (Clinical research ed.),
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
December 1990, The Malaysian journal of pathology,
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
August 1989, The Journal of hospital infection,
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
November 1988, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
February 2009, Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine],
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
December 1992, FEMS microbiology letters,
N Mangeney, and A Bakkouch, and J L Pons, and C Dupeyron, and P Niel, and G Leluan
December 2018, Journal of global antimicrobial resistance,
Copied contents to your clipboard!