Western blot analysis of rat pituitary antigens recognized by human antipituitary antibodies. 1995

S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
Department of Laboratory Medicine, Gunma University, School of Medicine, Maebashi, Japan.

Antipituitary antibodies have been reported to exist in sera of patients with autoimmune endocrine disorders. In order to investigate the pituitary antigens recognized by antipituitary antibodies, we studied the autoantigens in rat pituitary membrane and cytosolic fractions recognized by human antipituitary antibodies and anti-human pituitary hormone antibodies. Sera from 6 patients which showed positive antipituitary antibodies by immunofluorescence methods were studied. Each serum identified some proteins with 14.5, 22, 47, 49, 65, 84 and 97.5 kDa. Anti-GH antibodies and anti-PRL antibodies identified a positive band with 22 kDa, suggesting that anti-GH and anti-PRL antibodies may be present in patients' sera. These results indicate that Western blot analysis of rat pituitary antigens recognized by human antipituitary antibodies is a useful method to elucidate the pathophysiology of autoimmune endocrine disorders.

UI MeSH Term Description Entries
D010902 Pituitary Gland A small, unpaired gland situated in the SELLA TURCICA. It is connected to the HYPOTHALAMUS by a short stalk which is called the INFUNDIBULUM. Hypophysis,Hypothalamus, Infundibular,Infundibular Stalk,Infundibular Stem,Infundibulum (Hypophysis),Infundibulum, Hypophyseal,Pituitary Stalk,Hypophyseal Infundibulum,Hypophyseal Stalk,Hypophysis Cerebri,Infundibulum,Cerebri, Hypophysis,Cerebrus, Hypophysis,Gland, Pituitary,Glands, Pituitary,Hypophyseal Stalks,Hypophyses,Hypophysis Cerebrus,Infundibular Hypothalamus,Infundibular Stalks,Infundibulums,Pituitary Glands,Pituitary Stalks,Stalk, Hypophyseal,Stalk, Infundibular,Stalks, Hypophyseal,Stalks, Infundibular
D010907 Pituitary Hormones Hormones secreted by the PITUITARY GLAND including those from the anterior lobe (adenohypophysis), the posterior lobe (neurohypophysis), and the ill-defined intermediate lobe. Structurally, they include small peptides, proteins, and glycoproteins. They are under the regulation of neural signals (NEUROTRANSMITTERS) or neuroendocrine signals (HYPOTHALAMIC HORMONES) from the hypothalamus as well as feedback from their targets such as ADRENAL CORTEX HORMONES; ANDROGENS; ESTROGENS. Hormones, Pituitary
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001323 Autoantibodies Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them. Autoantibody
D001324 Autoantigens Endogenous tissue constituents with the ability to interact with AUTOANTIBODIES and cause an immune response. Autoantigen,Autologous Antigen,Autologous Antigens,Self-Antigen,Self-Antigens,Antigen, Autologous,Antigens, Autologous,Self Antigen,Self Antigens
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
August 1983, Journal of immunology (Baltimore, Md. : 1950),
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
August 1988, Journal of the National Cancer Institute,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
October 1986, Infection and immunity,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
January 1994, Research in microbiology,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
June 1993, The Southeast Asian journal of tropical medicine and public health,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
May 1994, Annals of surgical oncology,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
December 1990, Journal of clinical microbiology,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
December 1986, Journal of clinical microbiology,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
November 1995, Yonsei medical journal,
S Yabe, and M Murakami, and K Maruyama, and H Miwa, and Y Fukumura, and S Ishii, and M Sugiura, and I Kobayashi
July 1998, The Journal of laboratory and clinical medicine,
Copied contents to your clipboard!