-transparent.png){kind=logo}
The behaviour of synthetic batches of beta-amyloid (beta A) 1-40 peptide in solution has been studied. The effects of beta A1-40 on a PC12 cell toxicity assay was dependent upon the time of preincubation of an aqueous solution of the peptide before application to the cells. Fibrillization of the beta A1-40, quantitatively assessed by the binding of Congo red to amyloid fibrils, also increased in a time dependent manner over the 168 h incubation period studied. The degree of Congo red binding, in the absence of any preincubation, differed between two synthetically distinct batches of the peptide. The rate of development of fibril formation during subsequent incubation also differed between the two batches and appeared to parallel the effects on cell viability. Infra-red spectroscopic analysis revealed beta-sheet formation for both batches and other more subtle conformational differences between the peptides. Electron microscope examination of the batches of beta A1-40 confirmed the difference in occurrence and development of fibrils. At high magnification, fibrils of both batches exhibited a helical structure. The results suggest that the development of neurotoxicity of beta A1-40 is related to the fibrillar state of the peptide.
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
May 2011,
Current protein & peptide science,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
November 1998,
Biochemistry and molecular biology international,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
December 2009,
Applied magnetic resonance,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
July 2017,
Molecular bioSystems,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
April 1992,
Neurochemical research,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
January 1996,
Proceedings of the National Academy of Sciences of the United States of America,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
August 2010,
Proceedings of the National Academy of Sciences of the United States of America,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
January 2005,
Brain pathology (Zurich, Switzerland),
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
April 2002,
Current topics in medicinal chemistry,
D R Howlett, and
K H Jennings, and
D C Lee, and
M S Clark, and
F Brown, and
R Wetzel, and
S J Wood, and
P Camilleri, and
G W Roberts
September 2007,
Current opinion in drug discovery & development,
