Transcapillary escape rate of albumin (TER) expressed as percentage decrease in specific activity of plasma albumin per hour was measured in 44 healthy male subjects, 10 young non-smokers (median age 24.0 years, range 21-33), 10 young smokers (30.5 years, range 27-36), 10 elderly non-smokers (64.0 years, range 51-75) and 14 elderly smokers (61.5 years, range 54-69). In addition urinary albumin excretion rates were measured in 15 of the elderly subjects. The subjects were investigated after overnight fasting and abstention from tobacco. Median TER was 7.33% (interquartile range 5.82-9.90) in the elderly smoking subjects. This value was significantly elevated as compared to elderly non-smokers, 4.47% (2.93-5.83), young non-smokers, 3.70% (2.24-6.30) and young smokers, 4.25% (2.80-4.44). A statistically non-significant (P = 0.064) tendency of a higher urinary albumin excretion rate was observed in elderly smokers. This agrees with other investigations showing slightly increased albuminuria in smokers compared with non-smokers. The differences were not related to differences in blood pressure, plasma cholesterol, weight, or abuse of alcohol in the elderly subjects. Diabetic subjects with an increased TER to levels comparable with levels in long-term smokers have increased urinary albumin excretion rates, which seems to be greater than in long-term smoking subjects. It is suggested that increased TER in long-term smokers may indicate a vascular dysfunction affecting either capillary permeability or endothelial surface area.