BIOMAT Database Logo BIOMAT Database Logo

Quantitative cytofluorimetric determination of cell membrane-associated large tumor antigen on SV40-transformed cells. 1995

R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Abteilung Virologie, Albert-Ludwigs-Universität, Freiburg, Germany.

The aim of this study was to quantitate the number of cell membrane-located SV40 large tumor antigen (large T) molecules of SV40-transformed cell lines by cytofluorimetric analysis. Five different SV40-transformed cell lines were labelled by either a biotin- or a fluorescein-conjugated monoclonal antibody, PAb1605, which is specific for the large T carboxyterminus. The conjugated-antibody fluorescence signals of the stained large T molecules of transformed cells were measured via cytofluorimetry. Comparison of the fluorescence signals of calibrated beads bearing a known number of fluorescein molecules to the signals of conjugated PAb1605 antibodies bound on microbeads to a defined number of IgG binding sites made it possible to determine the number of antibody-accessible large T molecules per SV40-transformed cell. The numbers (x10(-4)) found per cell were 1.0 (ELONA, hamster), 3.0 (VLM, mouse), 3.5 (mKSA, mouse), 11 (C57SV, mouse), and 5.5 (SV80, human), respectively. Thus, the technique described allows a precise quantitation of surface-exposed, antibody-accessible viral antigen expression.

UI MeSH Term Description Entries
D002461 Cell Line, Transformed Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals. Transformed Cell Line,Cell Lines, Transformed,Transformed Cell Lines
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000952 Antigens, Polyomavirus Transforming Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle. Polyomavirus Large T Antigens,Polyomavirus Middle T Antigens,Polyomavirus Small T Antigens,Polyomavirus T Proteins,Polyomavirus Transforming Antigens,Polyomavirus Tumor Antigens,SV40 T Antigens,SV40 T Proteins,Simian Sarcoma Virus Proteins,Polyomaviruses Large T Proteins,Polyomaviruses Middle T Proteins,Polyomaviruses Small T Proteins,Antigens, Polyomavirus Tumor,Antigens, SV40 T,Proteins, Polyomavirus T,Proteins, SV40 T,T Antigens, SV40,T Proteins, Polyomavirus,T Proteins, SV40,Transforming Antigens, Polyomavirus,Tumor Antigens, Polyomavirus
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Surface-associated large tumor antigen is mainly detected in proliferating SV40-transformed cells.
February 1990, Cell biology international reports,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Association of SV40 large tumor antigen and cellular proteins on the surface of SV40-transformed mouse cells.
July 1982, Virology,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Surface antigen(s) of SV40-transformed tumor cells.
May 1970, Virology,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Effect of nuclear localization of large tumor antigen on growth potential of SV40-transformed cells.
April 1981, Virology,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Expression of SV40 tumor antigen in SV40 transformed teratocarcinoma-derived cell lines.
August 1980, Journal of cellular physiology,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
SV40-transformed cells express SV40 T antigen-related antigens on the cell surface.
January 1981, Virology,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Detection of a complex of SV40 large tumor antigen and 53K cellular protein on the surface of SV40-transformed mouse cells.
January 1982, Journal of cellular biochemistry,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Regulation of SV40 tumor antigen in transformed mouse 3T3 cells.
January 1975, Cold Spring Harbor symposia on quantitative biology,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.
February 1982, Cell,
R D Hess, and M Kuther, and C Haessler, and S Paetzold, and D G Braun, and G Brandner
Topoisomerase activity associated with SV40 large tumor antigen.
April 1993, Nucleic acids research,
Copied contents to your clipboard!