-transparent.png){kind=logo}
Plasmid DNA encoding proteins represent a convenient novel approach to vaccination. We have investigated this "genetic immunization" approach as a means to protect against herpes simplex virus (HSV) infection using a mouse zosteriform model that mimics several aspects of reactivated HSV infection of humans. After i.m. immunization with plasmid DNA-encoding glycoprotein B (gB), (pc-gB), 80% of BALB/c mice were completely protected and lesions were delayed in the remaining animals. Upon pc-gB vaccination, the animals developed both gB- and HSV-specific IgG Ab response and the isotype examination revealed a predominance of IgG2a. These mice also have low levels (1/16) of HSV-neutralizing Abs. Immune splenocytes obtained from pc-gB-immunized mice, when restimulated in vitro with HSV resulted in production of type 1 cytokines. Evidence for CD(8+)-mediated cytotoxic T lymphocyte response was equivocal. Protection could be adoptively transferred to nude mice recipients by CD4+ T cells from pc-gB-immunized mice but not by CD8+ T cells. Our results demonstrate that genetic immunization is a potent means of inducing protection against HSV and that the mechanism of immunity responsible for clearing virus from cutaneous sites is principally by CD4+ T cells. It is likely that these cells are Th1 cells because type 1 cytokines were the major cytokines detected upon in vitro Ag stimulation.
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
January 2002,
Journal of virology,
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
May 2009,
Vaccine,
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
July 1983,
Infection and immunity,
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
February 1989,
Journal of immunology (Baltimore, Md. : 1950),
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
February 1991,
Journal of virology,
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
January 1980,
Infection and immunity,
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
January 2012,
Neuroimmunomodulation,
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
May 1999,
Journal of immunology (Baltimore, Md. : 1950),
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
July 1993,
The Journal of general virology,
E Manickan, and
R J Rouse, and
Z Yu, and
W S Wire, and
B T Rouse
February 1977,
Nature,
