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A new plasmid vector, pCRP, allowing the expression of human recombinant monoclonal antibody Fab fragments fused with a bacterial acid phosphatase has been constructed. pCRP can accept heavy- and light-chain cDNAs cloned from combinatorial antibody libraries displayed on filamentous phages with the pCombIII system and is able to direct expression to soluble Fabs in which the carboxy-terminus of the heavy chain is fused to the amino-terminus of the mature PhoN nonspecific acid phosphatase of Providencia stuartii. Using the pCRP vector, we expressed two different human recombinant Fabs cloned from combinatorial libraries (one anti-tetenus toxoid and the other anti-HIV-1 gp120) fused with the acid phosphatase. In both cases chimeric antibodies were obtained which retained the antigen-binding ability and the enzymatic activity. Similar Fab-enzyme fusions can be successfully used, even unpurified, in enzyme immunoassays.
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
May 1999,
Clinical and diagnostic laboratory immunology,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
April 2004,
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
January 1999,
Journal of biochemistry,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
December 2003,
Journal of thrombosis and haemostasis : JTH,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
March 2010,
Infection and immunity,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
February 1994,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
March 1999,
Nature biotechnology,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
December 2002,
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
July 1989,
Journal of immunological methods,
R Burioni, and
P Plaisant, and
M L Riccio, and
G M Rossolini, and
R Santangelo, and
A Vannini, and
G Satta
May 2014,
Chembiochem : a European journal of chemical biology,
