BACKGROUND Recent studies conducted on the rat have demonstrated that the mesencephalic trigeminal nucleus (MTN) neurons, involved in the proprioceptive transmission, contain some neuroactive substances, including classical and amino acid neurotransmitters. In addition, there is evidence that serotonin could not act as a neurotransmitter at the first synaptic relay in the cat MTN. In the present study, we aimed to examine two other possible neurotransmitter systems, i.e., catecholamines and gamma-aminobutyric acid (GABA), and the relationships between GABA-immunoreactive (IR) neurons and tyrosine hydroxylase (TH)-IR axonal varicosities in the MTN of the cat. METHODS To ensure the localization of immunoreactive structures, the experiments were carried out at the light and electron microscopic level using single immunostaining for TH and GABA alone. The correlation between GABA-IR cell bodies and TH-IR fibers was investigated by means of double-labeling immunogold and peroxidase technique for GABA and TH. RESULTS Light microscopically, a few GABA-IR neurons were observed in the cat MTN. These small-size, labeled cells, most likely interneurons, were apposed to unstained large mesencephalic trigeminal cells. Most of the large nonreactive MTN neurons were closely surrounded by fine TH-IR varicose or nonvaricose fibers and dot-like structures, presumably nerve terminals. Under the electron microscope, TH-IR fibers were not seen in synaptic contact and only rarely appeared to be in close proximity to neuronal profiles of small GABAergic cells, which contained gold particles. CONCLUSIONS Taken together with earlier studies from other laboratories, the present findings suggest that GABAergic system might play an indirect role in the proprioceptive information processing in the cat MTN by interactions of GABA-immunoreactive neurons with the systems that control the transmission of selected sensory information. In contrast, the presence of TH-IR fibers in direct apposition to the majority of MTN neurons provide further evidence that presumed GABAergic interneurons have extensive interactions with catecholamine varicosities and raise the possibility that catecholamines could modify the transmission on these neurons.