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It is shown that leukocytes binding endotoxin of gram-negative bacteria via Fe-receptor mediated mechanism can be detected in blood smears after the treatment with antibodies to Re chemotype glycolipid conjugated with horse-radish peroxydase. After pretreatment of blood smears by a solution of endotoxin and then by conjugated antibodies to Re glycolipid some leukocytes binding endotoxin in vitro can be detected. It means that some reserves of endotoxin binding by leukocytes can be estimated by this immunomorphological method. The presence of such reserves is characteristic for healthy people and animals. Such reserves were enhanced in animals immunized with heated vaccine from Re Salmonella mutant. The reserves of endotoxin binding by leukocytes are absent in patients with salmonellosis, dysentery, meningitis, viral hepatitis A and B, peritonitis.
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M Iu Iakovlev
April 2000,
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
N K Permiakov, and
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April 1991,
Biulleten' eksperimental'noi biologii i meditsiny,
N K Permiakov, and
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M Iu Iakovlev
January 1987,
The Yale journal of biology and medicine,
N K Permiakov, and
I A Anikhovskaia, and
A N Krupnik, and
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M Iu Iakovlev
March 1977,
The Journal of laboratory and clinical medicine,
N K Permiakov, and
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February 2008,
Virus research,
N K Permiakov, and
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M Iu Iakovlev
May 1978,
The Journal of cell biology,
N K Permiakov, and
I A Anikhovskaia, and
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M Iu Iakovlev
March 1980,
Blut,
N K Permiakov, and
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November 1976,
Experimental cell research,
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M Iu Iakovlev
December 1991,
Nihon Ika Daigaku zasshi,
N K Permiakov, and
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December 1984,
Infection and immunity,
