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Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as beta-galactosidase activity. Transcription of the crystal protein gene fusion began immediately before the end of exponential growth and continued into stationary phase in both B. sphaericus and Bacillus subtilis but accompanied exponential-phase growth in Escherichia coli. Expression of this fusion was severely delayed in a B. subtilis spo0A mutant and decreased relative to the wild type in a B. subtilis spoIIAC background. beta-Galactosidase activity from the 100-kDa toxin gene fusion was restricted to early exponential phase in B. sphaericus, but in B.subtilis it continued into late exponential phase. Expression was about eightfold lower in B. sphaericus than B. subtilis suggesting an element of negative control in the native host.
H K Ahmed, and
W J Mitchell, and
F G Priest
April 2006,
Journal of molecular biology,
H K Ahmed, and
W J Mitchell, and
F G Priest
September 2006,
Bioscience, biotechnology, and biochemistry,
H K Ahmed, and
W J Mitchell, and
F G Priest
August 1992,
Journal of bacteriology,
H K Ahmed, and
W J Mitchell, and
F G Priest
February 2009,
Canadian journal of microbiology,
H K Ahmed, and
W J Mitchell, and
F G Priest
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Applied and environmental microbiology,
H K Ahmed, and
W J Mitchell, and
F G Priest
January 1989,
Applied and environmental microbiology,
H K Ahmed, and
W J Mitchell, and
F G Priest
November 1995,
Applied and environmental microbiology,
H K Ahmed, and
W J Mitchell, and
F G Priest
April 1996,
Gene,
H K Ahmed, and
W J Mitchell, and
F G Priest
October 1993,
Applied and environmental microbiology,
H K Ahmed, and
W J Mitchell, and
F G Priest
July 2006,
Journal of medical entomology,
