Biomaterial Database

A combined retrograde tracer and GABA-immunocytochemical study of the projection from nucleus interpositus posterior to the posterior lobe C2 zone of the cat cerebellum. 1995

J Kolston, and R Apps, and J R Trott

Department of Physiology, School of Medical Sciences, University of Bristol, UK.

The extent to which the cells of origin of the cerebellar nucleocortical pathway are immunopositive for gamma-aminobutyric acid (GABA) was investigated in four cats using retrograde labelling of nucleocortical neurons in combination with immunocytochemistry. Neurons were retrogradely labelled by injection of fluorescent (coumarin)-tagged latex microspheres into the c2 zone in the rostral part of the paramedian lobule. The zone was identified electrophysiologically by the characteristics of the climbing fibre responses evoked on the cerebellar surface by percutaneous stimulation applied to the left and right forepaws in pentobarbitone-anaesthetized animals. Sections of the cerebellum containing the retrogradely labelled neurons were processed for GABA immunocytochemistry using a fluorescent (rhodamine)-tagged immunoglobulin. When viewed with epifluorescence microscopy and appropriate filter blocks the retrogradely labelled nucleocortical neurons could be visualized in the same sections as the GABA-immunopositive neurons. Almost all of a total of 254 labelled nucleocortical neurons were located in nucleus interpositus posterior, where a total of 711 GABAergic neurons were also found. None of these cells contained coumarin-tagged beads and displayed immunoreactivity for GABA (i.e. none was double-labelled). When compared by area of their cell body, the nucleocortical and GABA-immunopositive neurons appeared to form two partially overlapping populations. The mean cell area of the nucleocortical neurons was 620 +/- 233 microns2 (SD), whereas the GABA-immunopositive neurons were much smaller, with a mean cell area of 220 +/- 115 microns2. The results suggest that GABA does not play a major role in the nucleocortical pathway to the c2 zone of the rostral paramedian lobule of the cat cerebellum.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D009434	Neural Pathways	Neural tracts connecting one part of the nervous system with another.	Neural Interconnections,Interconnection, Neural,Interconnections, Neural,Neural Interconnection,Neural Pathway,Pathway, Neural,Pathways, Neural
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002452	Cell Count	The number of CELLS of a specific kind, usually measured per unit volume or area of sample.	Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D002531	Cerebellum	The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.	Cerebella,Corpus Cerebelli,Parencephalon,Cerebellums,Parencephalons
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005680	gamma-Aminobutyric Acid	The most common inhibitory neurotransmitter in the central nervous system.	4-Aminobutyric Acid,GABA,4-Aminobutanoic Acid,Aminalon,Aminalone,Gammalon,Lithium GABA,gamma-Aminobutyric Acid, Calcium Salt (2:1),gamma-Aminobutyric Acid, Hydrochloride,gamma-Aminobutyric Acid, Monolithium Salt,gamma-Aminobutyric Acid, Monosodium Salt,gamma-Aminobutyric Acid, Zinc Salt (2:1),4 Aminobutanoic Acid,4 Aminobutyric Acid,Acid, Hydrochloride gamma-Aminobutyric,GABA, Lithium,Hydrochloride gamma-Aminobutyric Acid,gamma Aminobutyric Acid,gamma Aminobutyric Acid, Hydrochloride,gamma Aminobutyric Acid, Monolithium Salt,gamma Aminobutyric Acid, Monosodium Salt
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia