-transparent.png){kind=logo}
We describe a new specific method for measuring lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid by thin-layer chromatography, with use of a hydrogen flame ionization detector. After extraction and acetone precipitation to isolate surface-active lecithin, the phospholipids are separated on a thin rod of refractory and chemically stable material, having an outer coating of a bonded, sintered partition medium. Two solvent systems are used to develop the chromatograms, chloroform/methanol/water to separate lecithin and sphingomyelin, tetrahydrofuran/methylal/methanol for phosphatidylglycerol. Then the rod is passed through an hydrogen flame; the resulting ions produced generate a current, which is amplified and fed to a potentiometric recorder. The height of integral curves was proportional to the area under each peak. For quantitation we used an internal standard (lysolecithin in the first system. phosphatidyldimethylethanolamine in the second). The method requires less than 5 mL of amniotic fluid; results are available within 6 h.
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
August 1971,
Rinsho byori. The Japanese journal of clinical pathology,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
March 1980,
Clinical chemistry,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
April 1984,
The Journal of reproductive medicine,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
July 1981,
Obstetrics and gynecology,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
March 1983,
Clinical chemistry,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
October 1980,
Lipids,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
January 1982,
Clinical chemistry,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
October 2002,
Journal of chromatography. A,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
May 1973,
Scandinavian journal of clinical and laboratory investigation,
A Martin-Ponthieu, and
N Porchet, and
J C Fruchart, and
G Sezille, and
P Dewailly, and
X Codaccioni, and
M Delecour
April 2010,
Biomedical chromatography : BMC,
