Biomaterial Database

Early ultrastructural changes in the rat testis after ductuli efferentes ligation. 1979

E Anton

The early effects of ligation of the ductuli efferentes on testicular ultrastructure were studied in adult rats. As soon as 24 hours after the operation a large number of vacuoles appeared in the cytoplasm of Sertoli cells; vacuolization persisted for as long as 9 days, but localized in the apical part of the cells. Giant multinucleated cells containing annular clumps of chromatin were observed 1 day after ligation. The number of giant cells increased steadily as well as the alterations shown by their chromatin and cytoplasm. Isolated young spermatids also suffered morphologic changes that varied from almost normality to absolute necrosis. The thickness of the limiting membrane increased, as well as the number of cell layers. Deep infoldings, apparently caused by shrinkage of the tubules, were also noticed. Spermatogonias, spermatocytes I, mature spermatids, and spermatozoa did not present abnormalities. The interstitial tissue and the Leydig cells also appeared normal. It is concluded that ductuli efferentes ligation affects primarily the Sertoli cells; the formation of giant cells and the alterations shown by early spermatids and basement membrane are probably not specific but consequences of the injury to the Sertoli cells.

UI	MeSH Term	Description	Entries
D007985	Leydig Cells	Steroid-producing cells in the interstitial tissue of the TESTIS. They are under the regulation of PITUITARY HORMONES; LUTEINIZING HORMONE; or interstitial cell-stimulating hormone. TESTOSTERONE is the major androgen (ANDROGENS) produced.	Interstitial Cells, Testicular,Leydig Cell,Testicular Interstitial Cell,Testicular Interstitial Cells,Cell, Leydig,Cell, Testicular Interstitial,Cells, Leydig,Cells, Testicular Interstitial,Interstitial Cell, Testicular
D008026	Ligation	Application of a ligature to tie a vessel or strangulate a part.	Ligature,Ligations,Ligatures
D008297	Male		Males
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012708	Sertoli Cells	Supporting cells projecting inward from the basement membrane of SEMINIFEROUS TUBULES. They surround and nourish the developing male germ cells and secrete the ANDROGEN-BINDING PROTEIN and hormones such as ANTI-MULLERIAN HORMONE. The tight junctions of Sertoli cells with the SPERMATOGONIA and SPERMATOCYTES provide a BLOOD-TESTIS BARRIER.	Sertoli Cell,Cell, Sertoli,Cells, Sertoli
D013245	Sterilization, Reproductive	Procedures to block or remove all or part of the genital tract for the purpose of rendering individuals sterile, incapable of reproduction. Surgical sterilization procedures are the most commonly used. There are also sterilization procedures involving chemical or physical means.	Reproductive Sterilization,Voluntary Sterilization,Female Sterilization,Female Sterilization, Voluntary,Male Sterilization,Male Sterilization, Voluntary,Female Sterilizations,Female Sterilizations, Voluntary,Male Sterilizations,Male Sterilizations, Voluntary,Reproductive Sterilizations,Sterilization, Female,Sterilization, Male,Sterilization, Voluntary,Sterilizations, Female,Sterilizations, Male,Sterilizations, Reproductive,Sterilizations, Voluntary,Voluntary Female Sterilization,Voluntary Female Sterilizations,Voluntary Male Sterilization,Voluntary Male Sterilizations,Voluntary Sterilizations
D013737	Testis	The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.	Testicles,Testes,Testicle
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus