-transparent.png){kind=logo}
Evidence is presented that minigastrin is the C-terminal tetradecapeptide amide of gastrin and not the tridecapeptide amide as previously reported. Synthesis of the tetradecapeptide amide sequence, Trp-Leu-[Glu]5-Ala-Tyr-Gly-Trp-Met-Asp-Phe-Nh2, was achieved by a series of fragment couplings which were mediated by the dicyclohexylcarbodiimide procedure in presence of either N-hydroxysuccinimide or 1-hydroxybenzotriazole. Purification of all intermediate fragments, and of the final protected tetradecapeptide amide, was by Sephadex LH-20 chromatography. Removal of the protecting groups was effected by treatment with 90% trifluoroacetic acid in the presence of a large excess of scavengers. Purification by ion-exchange chromatography afforded the pure tetradecapeptide amide. This material had full physiological activity.
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
February 2019,
Journal of natural products,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
May 2020,
Journal of natural products,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
October 1986,
FEBS letters,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
January 1990,
Biopolymers,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
July 2023,
EJNMMI research,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
October 1987,
Biological chemistry Hoppe-Seyler,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
September 1974,
Gut,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
January 1981,
Digestion,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
April 2000,
Organic letters,
R A Gregory, and
H J Tracy, and
J I Harris, and
M J Runswick, and
S Moore, and
G W Kenner, and
R Ramage
January 2015,
Nuclear medicine review. Central & Eastern Europe,
