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Two different allele-specific suppressor mutants of the fission yeast Schizosaccharomyces pombe produce opal (UGA) suppressor tRNAs. This was shown by the use of a new in vitro assay for eukaryotic nonsense suppression: a wheat germ extract is programmed with rabbit globin mRNAs and the readthrough products are studied. alpha-Globin is elongated upon addition of ochre (UAA) suppressor tRNAs, whereas beta-globin yields a readthrough product with opal suppressor tRNAs. This simple and very sensitive assay allowed the purification of the opal suppressor tRNA from S. pombe strain sup3-e. The pure tRNA can be aminoacylated with serine; thus, we conclude that this suppressor tRNA inserts serine in response to the UGA termination codon of pure rabbit beta-globin mRNA.
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
June 1979,
Nucleic acids research,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
May 1979,
Molecular & general genetics : MGG,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
April 1988,
Genome,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
November 1982,
Nature,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
October 1982,
Proceedings of the National Academy of Sciences of the United States of America,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
August 1981,
Cell,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
January 1984,
Cold Spring Harbor symposia on quantitative biology,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
January 1999,
Methods in molecular biology (Clifton, N.J.),
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
January 2012,
Scientifica,
J Kohli, and
T Kwong, and
F Altruda, and
D Söll, and
G Wahl
January 1974,
Biochemical and biophysical research communications,
