-transparent.png){kind=logo}
A simple procedure for the preparation of deglycosylated avidin is described. Commercially obtained avidin was treated with a mixed microbial culture. The cells were capable of growing on the oligosaccharide residues, but generally ignored the polypeptide portion of the egg white glycoprotein. The resultant deglycosylated avidin retained its biotin-binding characteristics. The major bacterial strain (strain BECH080), responsible for the deglycosylation, was isolated. On the basis of elementary biochemical tests, fatty acid, and phenotypic analyses, the isolate was identified as a strain of Flavobacterium meningosepticum. The primary enzymatic activity that caused the removal of the oligosaccharide residues of avidin appeared to be similar to endoglycosidase F.
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
July 1975,
The Journal of biological chemistry,
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
January 1994,
Journal of molecular biology,
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
September 1941,
Science (New York, N.Y.),
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
September 1984,
Journal of molecular biology,
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
October 1978,
The Journal of biological chemistry,
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
August 1976,
The Biochemical journal,
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
January 1992,
Methods in molecular biology (Clifton, N.J.),
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
January 1992,
Methods in molecular biology (Clifton, N.J.),
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
February 1971,
The Journal of biological chemistry,
E A Bayer, and
F De Meester, and
T Kulik, and
M Wilchek
January 1998,
Methods in molecular biology (Clifton, N.J.),
