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An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgG antibodies to purified sheep hydatic cyst fluid antigen in 56 sera of confirmed cases of hydatidosis. The cut-off value was determined using serum samples from 80 healthy persons, employing two serum dilutions (1:100 and 1:500) with two and three standard deviations (SD). This assay was compared with the indirect hemagglutination test (IHAT). The sensitivity of ELISA-IgG was 94.3% for hepatic cysts and 92.9% for pulmonary cysts, whereas the values for IHAT were 77.1 and 64.3% respectively. According to Mac Nemar test, both method presented statistical significance (p < 0.05). In order to find out the specificity, additional 70 serum samples from individuals with other parasitoses, such as cysticercosis (30), trichinosis (26) and fascioliasis (14) were also tested. IHAT presented a specificity of 92.7% and for ELISA-IgG the specificity using a cut-off of mean + 3 SD was 99.3 and 100.0% with sera dilution of 1:100 and 1:500 respectively when a cut-off of mean + 2 SD was considered, we found a specificity of 91.3 and 97.3% for 1:100 and 1:500 dilutions. The use of ELISA-IgG and purified antigen in the diagnosis of human hydatidosis is discussed.
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
January 1996,
Boletin chileno de parasitologia,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
January 1984,
Pathologie-biologie,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
January 1991,
Enfermedades infecciosas y microbiologia clinica,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
January 1994,
Revista de sanidad e higiene publica,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
November 1994,
Zhonghua yi xue za zhi = Chinese medical journal; Free China ed,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
January 1985,
Revista de igiena, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
January 2001,
Journal of clinical laboratory analysis,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
March 1975,
The American journal of tropical medicine and hygiene,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
January 2013,
Iranian journal of public health,
C Contreras M, and
S Gallo, and
P Salinas, and
J Sapunar, and
L Sandoval, and
F Solís
December 1988,
European journal of epidemiology,
