Biomaterial Database

Two cDNA clones encoding 14-3-3 homologs from tomato fruit. 1995

B Laughner, and S D Lawrence, and R J Ferl

Department of Horticultural Sciences, University of Florida, Gainesville 32611, USA.

Two tomato cDNA clones with homology to the 14-3-3 family of proteins were identified through immunoscreening a ripening tomato fruit library (Clontech). These two clones share approx. 71% identity at the nucleotide level and 84% identity at the deduced amino acid level, with radical amino acid substitutions clustering at the acidic carboxy-terminus. Southern hybridization data indicate that each clone represents a unique gene. Distinct transcript accumulation patterns during tomato fruit ripening together with the homology to brain regulatory proteins suggest potential involvement in fruit development.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012689	Sequence Homology, Nucleic Acid	The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.	Base Sequence Homology,Homologous Sequences, Nucleic Acid,Homologs, Nucleic Acid Sequence,Homology, Base Sequence,Homology, Nucleic Acid Sequence,Nucleic Acid Sequence Homologs,Nucleic Acid Sequence Homology,Sequence Homology, Base,Base Sequence Homologies,Homologies, Base Sequence,Sequence Homologies, Base
D014446	Tyrosine 3-Monooxygenase	An enzyme that catalyzes the conversion of L-tyrosine, tetrahydrobiopterin, and oxygen to 3,4-dihydroxy-L-phenylalanine, dihydrobiopterin, and water. EC 1.14.16.2.	Tyrosine Hydroxylase,3-Monooxygenase, Tyrosine,Hydroxylase, Tyrosine,Tyrosine 3 Monooxygenase
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D048948	14-3-3 Proteins	A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.	14-3-3 Protein,14-3-3 Protein, beta Isoform,14-3-3 Protein, epsilon Isoform,14-3-3 Protein, eta Isoform,14-3-3 Protein, gamma Isoform,14-3-3 Protein, tau Isoform,14-3-3 Protein, zeta Isoform,14-3-3 beta Protein,14-3-3 epsilon Protein,14-3-3 eta Protein,14-3-3 gamma Protein,14-3-3 tau Protein,14-3-3 zeta Protein,14-3-3beta Protein,14-3-3epsilon Protein,14-3-3eta Protein,14-3-3gamma Protein,14-3-3tau Protein,14-3-3zeta Protein,Brain 14-3-3 Protein,Protein 14-3-3,14 3 3 Protein,14 3 3 Protein, beta Isoform,14 3 3 Protein, epsilon Isoform,14 3 3 Protein, eta Isoform,14 3 3 Protein, gamma Isoform,14 3 3 Protein, tau Isoform,14 3 3 Protein, zeta Isoform,14 3 3 Proteins,14 3 3 beta Protein,14 3 3 epsilon Protein,14 3 3 eta Protein,14 3 3 gamma Protein,14 3 3 tau Protein,14 3 3 zeta Protein,14 3 3beta Protein,14 3 3epsilon Protein,14 3 3eta Protein,14 3 3gamma Protein,14 3 3tau Protein,14 3 3zeta Protein,14-3-3 Protein, Brain,Brain 14 3 3 Protein,Protein, 14-3-3tau,eta Protein, 14-3-3
D018076	DNA, Complementary	Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.	Complementary DNA,cDNA,cDNA Probes,Probes, cDNA