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1. The purpose of this study was to determine whether physiological changes in extracellular free [K+] cause significant changes in the Na(+)-K+ pump rate and intracellular free [Na+]. 2. The Na(+)-K+ pump rate was measured in human lymphocytes by determining ouabain-sensitive 86Rb+ influx at several concentrations of K+. The Na(+)-K+ pump rate increased within the physiological range of extracellular free [K+] (K1/2 = 1.5 mmol/l). 3. To test the hypothesis that elevation of extracellular free [K+] reduces intracellular free [Na+] rapidly, which in turn then slows the pump rate during experimental incubations, lymphocyte intracellular free [Na+] was measured using the fluorochrome sodium-binding benzofuran isophthalate. With larger elevations of extracellular free [K+], intracellular free [Na+] dropped more rapidly. Thus previous discrepancies among determinations of K1/2 may be the result of variations in incubation times, which can skew the pump rates measured during incubations in various extracellular free [K+] values. Steady-state intracellular free [Na+] varied inversely with extracellular free [K+].
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
February 1983,
Clinical science (London, England : 1979),
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
March 1974,
The Journal of general physiology,
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
June 1987,
Hypertension (Dallas, Tex. : 1979),
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
January 1971,
Naunyn-Schmiedebergs Archiv fur Pharmakologie,
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
December 1990,
Sheng li xue bao : [Acta physiologica Sinica],
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
January 1961,
Acta medica Academiae Scientiarum Hungaricae,
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
January 1981,
The Journal of physiology,
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
February 1982,
Rinsho byori. The Japanese journal of clinical pathology,
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
January 1986,
Scandinavian journal of clinical and laboratory investigation. Supplementum,
G D Webb, and
E A Taylor, and
V M Oh, and
S B Yeo, and
L L Ng
July 2008,
Physical biology,
