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Recombinational effects of the antimutator allele tsL42 of gene 43 of phage T4, encoding DNA polymerase, were studied in crosses between rIIB mutants. Recombination under tsL42-restricted conditions differed from the normal one in several respects: (1) basic recombination was enhanced, especially within very short distances; (2) mismatch repair tracts were shortened, while the contribution of mismatch repair to recombination was not changed; (3) marker interference at very short distances was augmented. We infer that the T4 DNA polymerase is directly involved in mismatch repair, performing both excision of a nonmatched single strand (by its 3'-->5' exonuclease) and filling the resulting gap. A pathway for the mismatch repair was substantiated; it includes sequential action of endo VII (gp49)-->3'-->5' exonuclease (gp43)-->DNA polymerase (gp43)-->DNA ligase (gp30). It is argued that the marker interference at very short distances may result from the same sequence of events during the final processing of recombinational intermediates.
V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
October 1998,
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V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
April 1998,
Genetics,
V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
January 1998,
Annual review of genetics,
V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
September 1976,
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V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
May 1975,
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V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
April 1998,
Genetics,
V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
December 1995,
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V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
February 1994,
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V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
September 1978,
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V P Shcherbakov, and
L A Plugina, and
E A Kudryashova
September 1990,
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