Nuclear chromatin decondensation (NCD) of the human spermatozoa was induced by 1% sodium dodecyl sulphate (SDS). NCD of the spermatozoa induced in healthy and fertile men was significantly stronger and at higher rates than that in infertile men. In 1% SDS with 6 mM zinc chelating EDTA, metallothionein (MT) significantly enhanced NCD in a healthy man. In contrast MT alone significantly inhibited sperm NCD. Sperm NCD rate induced by 1% SDS in 10 infertile men was significantly inhibited by adding 75 or 750 micrograms ml-1 of MT. By adding 1.5 mM zinc, MT at concentrations of 0.75, 7.5, 75, or 750 micrograms ml-1, enhanced the inhibitory effect of 1.5 mM zinc. This suggested that thiols in the MT could, when liberated from zinc by zinc-chelating EDTA, induce sperm decondensation by cleaving stabilizing S-S bridges and that zinc bound to MT could exert a chromatin stabilizing effect mediated by the zinc dependent type of chromatin stability. The present study suggested that zinc bound to MT, which is secreted mainly from the prostate gland, is one factor that contributes to the chromatin stabilizing effect of human prostatic fluid.