Biomaterial Database

Limited heterogeneity in T-cell receptor V beta chain gene expression in arthritic joints of BUB/BnJ (H-2q) mice--a T-cell receptor V beta a strain. 1995

T M Haqqi, and X M Qu, and S Banerjee

Department of Medicine Case Western Reserve University, Cleveland, Ohio 44106-4946, USA.

We have earlier shown that T-cells in arthritic joints and LNs of B10.Q mice (H-2q, TCR V beta b) use a restricted number of TCR V beta chain genes (V beta 6, 8, 9). In the present study, we have investigated the TCR V beta chain gene expression in arthritic joints and LN of BUB/BnJ mice (H-2q, TCR V beta a). Mice were immunized with [table: see text] chicken type-II collagen, and arthritic joints and draining LNs were removed at the onset of arthritis and the TCR V beta chain gene expression was studied by PCR. A restricted usage of TCR V beta was observed in both the tissues. A dominant usage of TCR V beta 4, 7, and 15 was found in the LNs while TCR V beta 3 and 10 were predominantly expressed in arthritic joints in the majority of the arthritic mice (5/7). Our results indicate that (a) in H-2q mice with CIA there is a restricted usage of TCR V beta chain genes regardless of the TCR V beta genotype; and (b) in the absence of TCR V beta 8 and 9, TCR V beta 3 and 10 are predominantly used by joint-infiltrating T-cells.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D006183	H-2 Antigens	The major group of transplantation antigens in the mouse.	H2 Antigens,Antigens, H-2,Antigens, H2,H 2 Antigens
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001169	Arthritis, Experimental	ARTHRITIS that is induced in experimental animals. Immunological methods and infectious agents can be used to develop experimental arthritis models. These methods include injections of stimulators of the immune response, such as an adjuvant (ADJUVANTS, IMMUNOLOGIC) or COLLAGEN.	Adjuvant Arthritis,Arthritis, Adjuvant-Induced,Arthritis, Collagen-Induced,Arthritis, Adjuvant,Collagen Arthritis,Arthritides, Collagen,Arthritis, Collagen,Collagen Arthritides,Collagen-Induced Arthritides,Collagen-Induced Arthritis
D001327	Autoimmune Diseases	Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.	Autoimmune Disease,Disease, Autoimmune,Diseases, Autoimmune
D015333	Gene Rearrangement, beta-Chain T-Cell Antigen Receptor	Ordered rearrangement of T-cell variable gene regions coding for the beta-chain of antigen receptors.	T-Cell Antigen Receptor beta-Chain Gene Rearrangement,T-Lymphocyte Antigen Receptor beta-Chain Gene Rearrangement,Gene Rearrangement, beta-Chain T Cell Antigen Receptor,T Cell beta-Chain Gene Rearrangement,T Lymphocyte beta-Chain Gene Rearrangement,Gene Rearrangement, beta Chain T Cell Antigen Receptor,T Cell Antigen Receptor beta Chain Gene Rearrangement,T Cell beta Chain Gene Rearrangement,T Lymphocyte Antigen Receptor beta Chain Gene Rearrangement,T Lymphocyte beta Chain Gene Rearrangement
D016176	T-Lymphocyte Subsets	A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.	T-Cell Subset,T-Cell Subsets,T-Lymphocyte Subset,Subset, T-Cell,Subset, T-Lymphocyte,Subsets, T-Cell,Subsets, T-Lymphocyte,T Cell Subset,T Cell Subsets,T Lymphocyte Subset,T Lymphocyte Subsets
D016693	Receptors, Antigen, T-Cell, alpha-beta	T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.	Antigen Receptors, T-Cell, alpha-beta,T-Cell Receptors alpha-Chain,T-Cell Receptors beta-Chain,T-Cell Receptors, alpha-beta,TcR alpha-beta,Antigen T Cell Receptor, alpha Chain,Antigen T Cell Receptor, beta Chain,Receptors, Antigen, T Cell, alpha beta,T Cell Receptors, alpha beta,T-Cell Receptor alpha-Chain,T-Cell Receptor beta-Chain,T-Cell Receptor, alpha-beta,T Cell Receptor alpha Chain,T Cell Receptor beta Chain,T Cell Receptor, alpha beta,T Cell Receptors alpha Chain,T Cell Receptors beta Chain,TcR alpha beta,alpha-Chain, T-Cell Receptor,alpha-Chain, T-Cell Receptors,alpha-beta T-Cell Receptor,alpha-beta T-Cell Receptors,alpha-beta, TcR,beta-Chain, T-Cell Receptor,beta-Chain, T-Cell Receptors
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus