-transparent.png){kind=logo}
Using the patch-clamp technique, we determined that beta-scorpion toxin 2 from Centruroides noxius Hoffmann decreased whole-cell n-type K+ currents in human peripheral blood lymphocytes, with a half blocking concentration of approx. 5 microM. Toxin-2-accelerated inactivation, however, did not influence the kinetics of activation of the K+ conductance. The percentage increase in K+ channel inactivation rate and the degree of drug-induced block was independent of membrane potential. K+ channel block by Toxin 2 was instantaneous, not removable by washing with drug free extracellular solution. However, 10 mg/ml BSA in the bath lifted the toxin-induced block almost instantaneously and completely. Flow cytometric membrane potential measurements with the oxonol dye showed that Toxin 2 depolarizes human lymphocytes in concert with its K+ channel blocking effect.
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
March 1999,
Journal of molecular biology,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
May 1999,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
June 1989,
The Journal of general physiology,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
August 1997,
The Journal of membrane biology,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
September 1996,
Biochemical pharmacology,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
June 1986,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
May 1987,
Pflugers Archiv : European journal of physiology,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
August 1996,
Toxicon : official journal of the International Society on Toxinology,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
January 2004,
The Biochemical journal,
R Gáspár, and
L Bene, and
S Damjanovich, and
C Muñoz-Garay, and
E S Calderon-Aranda, and
L D Possani
June 1988,
The Journal of general physiology,
