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Transient expression experiments show that the maize GapA1 promoter exhibits a requirement for sequences contained within intron 1 and surrounding exon border regions for expression in maize Black Mexican Sweet cells. Maize GapA1-promoter constructs lacking intron 1 are inactive. Intron 1 and its exon border sequences, when reintroduced into constructs lacking introns, restore gene activity whereas intron 2 and its exon borders to not. The minimal promoter so defined encompasses roughly 250 bp upstream of the in vivo transcription start and appears also to include intron 1. An octameric sequence was identified in intron 1 of maize GapA1 which is similar to sequence motifs found in other maize introns known to increase transient expression. Partial restoration of gene expression in GapA1 constructs lacking intron 1 was achieved through insertion of the identified octameric sequence.
M Donath, and
R Mendel, and
R Cerff, and
W Martin
October 1997,
The Plant journal : for cell and molecular biology,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
October 1988,
Molecular and cellular biology,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
December 1990,
Plant molecular biology,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
March 1989,
Plant cell reports,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
January 1995,
Methods in molecular biology (Clifton, N.J.),
M Donath, and
R Mendel, and
R Cerff, and
W Martin
January 1991,
Molecular & general genetics : MGG,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
December 1989,
Plant physiology,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
July 1988,
Nucleic acids research,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
February 2002,
Insect molecular biology,
M Donath, and
R Mendel, and
R Cerff, and
W Martin
March 1993,
The Plant journal : for cell and molecular biology,
